Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Petersen, RJ , Hoffmann, CC , Zak, DH , Nilsson, I-EF , Audet, J & Andersen, HE 2025, 'Decreased aqueous exports of nitrogen, phosphorus and carbon after rewetting of a pump-drained fen', LuWQ2025. Land Use and Water Quality: Agriculture and the Environment , Aarhus, Denmark, 03/06/2025 - 06/06/2025.

Petersen, RJ , Hoffmann, CC , Zak, DH , Nilsson, I-EF , Audet, J & Andersen, HE 2025, 'Decreased aqueous exports of nitrogen, phosphorus and carbon after rewetting of pump-drained fen: Abstract 31', LuWQ2025. Land Use and Water Quality: Agriculture and the Environment , Aarhus, Denmark, 03/06/2025 - 06/06/2025.

Vogt, H & Poulsen, B, De danske stadsretter, 2025, Pictures, Video and sound recordings (digital). <https://podcasts.apple.com/dk/podcast/vogt-og-poulsen-de-danske-stadsretter/id1557854206?i=1000730539981>

Motzfeldt, HM & Næser, J 2025, De databeskyttelsesretlige behandlingsprincipper. in Almindelig databeskyttelsesret . 2 edn, Djøf Forlag, Kbh., pp. 129-164.

Carreto Picón, G , Zhou, PY , Zhang, Q & Iosifidis, A 2025 'DeepCoT: Deep Continual Transformers for Real-Time Inference on Data Streams'.

Skov, KB , Portillo-Perez, GA & Martinez, MM 2025, 'Deep eutectic solvent-assisted starch acetylation within stale bread particles to improve water resistance', International Journal of Biological Macromolecules, vol. 288, 138603. https://doi.org/10.1016/j.ijbiomac.2024.138603

Wu, H, Liao, Y, Hadi Mogavi, R, Hui, P & Zhou, PY 2025, Deepfake in the Metaverse: An Outlook Survey. in X Fang (ed.), HCI in Games : 7th International Conference, HCI-Games 2025, Held as Part of the 27th HCI International Conference, HCII 2025, Proceedings. Springer, Cham, Lecture Notes in Computer Science, vol. 15816 LNCS, pp. 253-267, 7th International Conference on HCI in Games, held as part of the 27th HCI International Conference, HCII 2025, Gothenburg, Sweden, 22/06/2025. https://doi.org/10.1007/978-3-031-92578-8_17

Sondergaard, K & EUSTAR Collaborators 2025, 'Deep hierarchical subtyping of multi-organ systemic sclerosis trajectories - a EUSTAR study', npj Digital Medicine, vol. 8, no. 1, 563. https://doi.org/10.1038/s41746-025-01962-y

Bjerge, K, Bodesheim, P & Karstoft, H 2025, Deep Image Clustering with Model-Agnostic Meta-Learning. in In Proceedings of the 20th International Joint Conference on Computer Vision. vol. 2, Imaging and Computer Graphics Theory and Applications - VISAPP 2025, Porto, pp. 286-297, The 20th International Joint Conference on Computer Vision, Imaging and Computer Graphics Theory and Applications - VISAPP 2025, Porto, Portugal, 26/02/2025. https://doi.org/10.5220/0013114600003912

Vestergaard, CD 2025, 'DEEP LEARNING-BASED CONE-BEAM COMPUTED TOMOGRAPHY CORRECTION FOR DOSE MONITORING AND ADAPTATION IN PROTON THERAPY', PhD, Aarhus University.

Spahr, A, Bernini, A, Ducouret, P, Baumgartner, C, Koren, JP, Imbach, L, Beniczky, S , Larsen, SA, Rheims, S, Fabricius, M, Seeck, M, Steinhoff, BJ, Beuchat, I, Dan, J, Atienza, DA, Bardyn, CE & Ryvlin, P 2025, 'Deep learning–based detection of generalized convulsive seizures using a wrist-worn accelerometer', Epilepsia, vol. 66, no. S3, pp. 53-63. https://doi.org/10.1111/epi.18406

Nazari, A, Najafi, SMY, Abbasi, R, Mohammad-Rahimi, H, Motie, P, Alamdari, MI, Hosseinzadeh, M, Pauwels, R & Schwendicke, F 2025, 'Deep learning for dentomaxillofacial cone-beam computed tomography image quality enhancement: A pilot study', Imaging Science in Dentistry, vol. 55, no. 3, pp. 271-279. https://doi.org/10.5624/isd.20250023

Bjerring, JC , Mainz, JT & Munch, LA 2025, 'Deep Learning Models and the Limits of Explainable Artificial Intelligence', Asian Journal of Philosophy, vol. 4, no. 1, 22. https://doi.org/10.1007/s44204-024-00238-8

Sun, Y, Qu, K, Corsi, GI, Anthon, C, Pan, X, Xiang, X, Jensen, LJ , Lin, L , Luo, Y & Gorodkin, J 2025, 'Deep learning models simultaneously trained on multiple datasets improve base-editing activity prediction', Nature Communications, vol. 16, no. 1, 9821. https://doi.org/10.1038/s41467-025-65200-5

Keck, SM, Barnhill, A, Smeele, SQ, Hsu, M, Dolson-Fazio, A, Bergler, C, Ball, GF, Krieg, CA & Odom, KJ 2025, 'Deep machine learning allows classification of male and female songbird songs in a species with highly variable repertoires', Behaviour, vol. 162, no. 10-11, pp. 709-743. https://doi.org/10.1163/1568539X-bja10324

Kokkinis, G , Iosifidis, A & Zhang, Q 2025, Deep Reinforcement Learning-Based Video-Haptic Radio Resource Slicing in Tactile Internet. in M Valenti, D Reed & M Torres (eds), ICC 2025 - IEEE International Conference on Communications. IEEE, IEEE International Conference on Communications, pp. 3821-3826, 2025 IEEE International Conference on Communications, ICC 2025, Montreal, Canada, 08/06/2025. https://doi.org/10.1109/ICC52391.2025.11160869

Allcock, AL, Amon, DJ, Bridges, AEH, Colaço, A, Escobar-Briones, E, Hilário, A, Howell, KL, Mestre, NC, Muller-Karger, FE, Priede, IG, Snelgrove, PVR, Sealey, KS, Xavier, JR, Addamo, AM, Amaro, T, Bandara, G, Bax, N, Braga-Henriques, A, Brandt, A, Brix, S, Cambronero-Solano, S, Cedeño – Posso, C, Copley, JT, Cordes, E, Cortés, J, Croquer, A, Cuvelier, D, Davies, JS, Durden, JM, Esquete, P, Foster, NL, Frutos, I, Gasbarro, R, Gates, AR, Gomes, M, Goodwin, LVM, Horton, T, Hourigan, T, Hoving, HJ, Jones, DOB, Joshi, S, Kingon, KC, Lörz, AN, Martins, A, Merten, V, Metaxas, A, Milligan, RJ, Molodtsova, TN, Morato, T, Morrissey, D, Naranjo-Elizondo, B, Narayanaswamy, BE, Olafsdottir, SH, Parimbelli, A, Peña, M, Piechaud, N, Ragnarsson, S, Ramalho, SP, Rodrigues, CF, Ross, RE, Saeedi, H, Santos, R, Schwing, PT, Serpa, T, Shantharam, AK, Stevenson, A, Yánez-Suárez, AB, Sutton, TT, Svavarsson, J, Taylor, ML, van der Grient, J & Zwerschke, N 2025, 'Deep-sea ecosystems of the North Atlantic Ocean: discovery, status, function and future challenges', Deep-Sea Research Part I: Oceanographic Research Papers, vol. 226, 104580. https://doi.org/10.1016/j.dsr.2025.104580

Nørgaard, M , Rusan, M , Kondrup, K , Sørensen, EMG , Weiss, S , Bjerre, MT , Fredsøe, J , Vang, S , Jensen, JB, De Laere, B, Grönberg, H, Borre, M, Lindberg, J & Sørensen, KD 2025, 'Deep targeted sequencing of circulating tumor DNA to inform treatment in patients with metastatic castration-resistant prostate cancer', Journal of Experimental & Clinical Cancer Research, vol. 44, no. 1, 120. https://doi.org/10.1186/s13046-025-03356-0

Kabatnik, S, Zheng, X, Pappas, G, Steigerwald, S, Padula, MP & Mann, M 2025, 'Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma', npj Precision Oncology, vol. 9, no. 1, 37. https://doi.org/10.1038/s41698-025-00819-7

Hu, J, Huang, C, Liu, L, Li, S, Song, F, Wells, JW & Wang, X 2025, 'Defect-Mediated Phase Transitions and Structural Dynamics in Single-Layer VSe₂', ACS Applied Materials and Interfaces, vol. 17, no. 33, pp. 47781-47789. https://doi.org/10.1021/acsami.5c15011

Berntorp, K, Mohammad, MA, Koul, S, Yndigegn, T, Bergman, S, Zwackman, S, Linder, R, Völz, S, Fröbert, O, Erlinge, D & Götberg, M 2025, 'Deferral of left main coronary artery revascularization via IVUS or coronary physiology - Long-term outcomes from the SWEDEHEART registry', International Journal of Cardiology, vol. 419, 132726. https://doi.org/10.1016/j.ijcard.2024.132726

Beyer, SA , Sonne, T , Kingo, OS & Krøjgaard, P 2025, 'Deferred Imitation: A 9-Month Revolution or an Emerging Ability at 6 Months?', Infancy, vol. 30, no. 6, e70053. https://doi.org/10.1111/infa.70053

Kronborg, CJS, Arp, DT, Bahij, R, Biancardo, SBN, Diness, LV, Engstrøm, KH, Fokdal, LU, Pedersen, BG, Havelund, B, Hvid, CA, Jakobsen, KL, Kirchheiner, K, Lutz, CM , Nyvang, L, Oggesen, BT, Petersen, SE, Poulsen, LØ, Rønde, HS , Schou, LK, Serup-Hansen, E, Steffensen, JH, Søndergaard, J, Szpejewska, J & Nissen, HD 2025, 'Defining and sparing sexual function-related organs at risk for rectal cancer radiotherapy', Acta Oncologica, vol. 64, pp. 1136-1142. https://doi.org/10.2340/1651-226X.2025.44011

Georgiadis, S, Ørnbjerg, LM, Michelsen, B, Kvien, TK, Shoae Kazemi, M, Závada, J, Pavelka, K, Glintborg, B, Loft, AG, Reich, A, Regierer, AC, Rodrigues, AM, Santos, MJ, Rutanen, J, Kuusalo, L, Ciurea, A, Nissen, MJ, Gudbjornsson, B, Pálsson, Ó, Rotar, Ž, Perdan Pirkmajer, K, Di Giuseppe, D, Østergaard, M & Hetland, ML 2025, 'Defining Bath Ankylosing Spondylitis Disease Activity Index Cut-off Values for Disease Activity States in a Multinational European Cohort of Patients With Axial Spondyloarthritis', ACR Open Rheumatology, vol. 7, no. 12, e70125. https://doi.org/10.1002/acr2.70125

Brown, KGM, Solomon, MJ, Koh, CE, Sutton, PA, Aguiar, S, Bezerra, TS, Clouston, HW, Desouza, A, Dozois, EJ, Ersryd, AL, Frizelle, F, Funder, JA, Garcia-Aguilar, J, Garfinkle, R, Glyn, T, Heriot, A, Kanemitsu, Y, Kong, CY, Kristensen, H, Malakorn, S, Mens, DM, Nilsson, PJ, Palmer, GJ, Pappou, E, Quinn, M, Quyn, AJ, Sahakitrungruang, C, Saklani, A, Solbakken, AM, Tiernan, JP, Verhoef, C & Steffens, D 2025, 'Defining Benchmarks for Pelvic Exenteration Surgery: A Multicentre Analysis of Patients With Locally Advanced and Recurrent Rectal Cancers', Annals of Surgery, vol. 282, no. 6, pp. 1118-1126. https://doi.org/10.1097/SLA.0000000000006348

Cirkovic, E & McDowell, J 2025, Defining military activities in outer space: the launching of the Iranian satellite Nour 1'. European Journal of International Law.

Larsdotter Zweygberg, A, Ahlqvist, VH & Magnusson, C 2025, 'Defining mode of delivery as “instrumental vaginal delivery”', American Journal of Obstetrics and Gynecology, vol. 232, no. 2, pp. e54-e55. https://doi.org/10.1016/j.ajog.2024.08.022

Dodds, WK, Barmuta, LA, Bernal, S, Corman, J, Harms, TK, Johnson, SL, Li, L, Fernandes Cunha, DG, Olden, JD, Riis, T, Silva, LCR, Stella, JC, Sullivan, P & Wohl, E 2025, 'Defining stream riparian zones across multidimensional environmental gradients', Journal of Environmental Quality, vol. 54, no. 6, pp. 1674-1697. https://doi.org/10.1002/jeq2.70080

Psychiatric Genomics Consortium Suicide Working Group & Mortensen, PB 2025, 'Defining suicidality phenotypes for genetic studies: perspectives of the Psychiatric Genomics Consortium Suicide Working Group', Molecular Psychiatry, vol. 30, no. 12, pp. 6144-6154. https://doi.org/10.1038/s41380-025-03271-y

Cirkovic, E 2025, Defining Sustainability for the Earth System and Outer Space. in Future uses of space: : Narrative Evidence for Science & Technology Advantage through linking Research and Policy .

AlZaim, I , Hassan, MN, Schröter, M, Mannino, L , Dragicevic, K , Sjogaard, MB , Festa, J , Dokshokova, L, Weinbrenner, S, Tardajos Ayllon, B, Hansen, B , Rasmussen, RK , Christensen, JN , Wagman, O, Schipper, R, Cai, M, Dheedene, W, Bohn, AB , Farup, J , Lin, L , Soraggi, S, Thorsen, AD, Bæk, A , Thomsen, HH, von Heesen, M, Conradi, L-C, Evans, P, Hagberg, CE, Heeren, J, Emont, M, Rosen, ED, Luttun, A, Etzerodt, A, Massier, L, Rydén, M, Mejhert, N, Blüher, M, Khodosevich, K, Fenton, RA, Sheikh, BN, Jessen, N, de Rooij, LPMH & Kalucka, J 2025 'Defining the vascular niche of human adipose tissue across metabolic states' bioRxiv. https://doi.org/10.1101/2024.09.22.610444

Global Cardiovascular Outcomes Consortium and in collaboration with ACNAP, ACVC, EACVI, EAPC, EAPCI, EHRA, ESC Committee for Young CV Professionals, ESC Registry Committee, HFA, ESC Patient Forum and these Working Groups: aorta and peripheral vascular diseases, atherosclerosis and vascular biology, cardiac cellular electrophysiology, cardiovascular pharmacotherapy, cardiovascular regenerative and restorative medicine, cardiovascular surgery, cellular biology of the heart, e-cardiology, myocardial function, pulmonary circulation and right ventricular function and thrombosis & Grove, EL 2025, 'Definitions of clinical study outcome measures for cardiovascular diseases: the European Unified Registries for Heart Care Evaluation and Randomized Trials (EuroHeart)', European Heart Journal, vol. 46, no. 2, pp. 190-214. https://doi.org/10.1093/eurheartj/ehae724

Borup, J 2025, De fire ædle sandheder. in lex.dk.

Guo, J, Li, P, Piccolo, F, Jie, Y, Jia, H, Cao, R, Jiao, S, Adelhelm, P & Xu, Y 2025, 'Degradation and Failure Mechanisms of Lithium/LiNi_xCo_yMn_1-x-yO₂ Batteries', ACS Energy Letters, vol. 10, no. 5, pp. 2318-2340. https://doi.org/10.1021/acsenergylett.4c03578

Bian, L, Zhao, Z, Wang, X, Sanei, H, Chappaz, A, Dong, J, Dong, Z, Xie, L, Schovsbo, NH, Goodarzi, F, Zhang, S & Zhao, W 2025, 'Degradation of organic matter by radioactive radiation in black shales: An overlooked modification of organic molecular structures', International Journal of Coal Geology, vol. 309, 104864. https://doi.org/10.1016/j.coal.2025.104864

Fitzpatrick, N 2025, 'Degrowth' and the implications of English language hegemony. in A Nelson (ed.), Routledge Handbook of Degrowth. Routledge, pp. 142-152. https://doi.org/10.4324/9781032650159-13

Kuhlmann, A 2025, Dehlholm, Kirsten. in MM Delgado & S Williams (eds), The Cambridge Encyclopedia of Stage Directors. Cambridge University Press, Cambridge . https://doi.org/10.1017/9781108115995

Petersen, KD, Welsh, MJ & Clausen, OR 2025, Dehnungsentwicklung in den über dem Kraka-Salzkissen liegenden Schichten, abgeleitet aus der stratigraphischen Rekonstruktion. in Geomechanische Kontrollen der Rissentwicklung in Kreide und Mergel in der dänischen Nordsee: Verstehen und Vorhersagen von Riss-Systemen. Springer Nature, pp. 155-181. https://doi.org/10.1007/978-3-031-81345-0_6

Youngblood, JP, Overgaard, J , Ørsted, M, Angilletta, MJ & VandenBrooks, JM 2025, 'Dehydration worsens heat tolerance of locusts and amplifies predicted impacts of climate change', Functional Ecology, vol. 39, no. 5, pp. 1194-1207. https://doi.org/10.1111/1365-2435.70038

Hanberg, P & Hougaard, DD 2025, 'De hyppigst forekomne sygdomme i balanceorganerne', Ugeskrift for Læger, vol. 187, no. 42. https://doi.org/10.61409/V03250234

Nielsen, KH 2025, 'DEI-fri forskning', Weekendavisen, vol. Sektion 4 (Ideer), pp. 5.

Hansen, GLM & Pikilak, I 2025, 'Dekolonisering af dansk-kalaallit samarbejde(r): Samrefleksioner over udstillingsprocessen omkring Inuit Dimensions i rum46', Kvinder, Køn & Forskning, vol. 38, no. 1, pp. 197-211. https://doi.org/10.7146/kkf.v38i1.156615

Ciampi, M, Sternad, J & Xia, Y 2025, Delayed-Input Multi-party Computation. in M Fischlin & V Moonsamy (eds), Applied Cryptography and Network Security - 23rd International Conference, ACNS 2025, Proceedings. Lecture Notes in Computer Science, vol. 15825, pp. 339–368. https://doi.org/10.1007/978-3-031-95761-1_12

Hu, L , van der Sluis, RM , Castelino, KB, Zhang, B-C, Ronit, A, Zillinger, T , Werner, M , Jørgensen, SE , Hansen, AL , Pedersen, A , Narita, R , Reinert, LS , Bundgaard, B , Holm, CK, Cobat, A, Casanova, J-L, Reggiori, F , Mari, M , Paludan, SR , Mogensen, TH & COVID Human Genetic Effort 2025, 'Deleterious variants in the autophagy-related gene RB1CC1/FIP200 impair immunity to SARS-CoV-2', Nature Communications, vol. 16, no. 1, 10618. https://doi.org/10.1038/s41467-025-65308-8

Baur, ST, Schulz, S, McCluskey, JB, Velázquez Gómez, JA, Angenent, LT & Molitor, B 2025, 'Deletion of aldehyde:ferredoxin oxidoreductase-encoding genes in Clostridium ljungdahlii results in changes in product spectrum with various carbon sources', Bioresource Technology, vol. 431, 132596. https://doi.org/10.1016/j.biortech.2025.132596

Esteva-Font, C , Zheng, X, Rudolphi, CF, Hansen, TPK, Hoorn, EJ & Fenton, RA 2025, 'Deletion of the prostaglandin EP4 receptor in the kidney tubule of mature mice alters kidney water handling only in males', American Journal of Physiology - Renal Physiology, vol. 328, no. 6, pp. F800-F814. https://doi.org/10.1152/ajprenal.00071.2025

Mikus, N, Kutlikova, HH, Christoph, E & Lamm, C 2025, 'Delineating testosterone's impact on model-based and model-free decision-making processes', Proceedings of the Royal Society B: Biological Sciences, vol. 292, no. 2060, 20252239. https://doi.org/10.1098/rspb.2025.2239

Lind-Holst, M, Hansen, D, Main, KM, Juul, A, Andersen, MS, Dunø, M, Rasmussen, ÅK, Jørgensen, N, Gravholt, CH & Berglund, A 2025, 'Delineating the psychiatric morbidity spectrum in congenital adrenal hyperplasia: a population-based registry study', The Journal of Clinical Endocrinology & Metabolism, vol. 110, no. 8, pp. 2147-2156. https://doi.org/10.1210/clinem/dgae780

Evans, M, Bonomo, P, Chan, PC, Chua, MLK, Eriksen, JG, Hunter, K, Jensen, K, Jones, TM, Laskar, SG, Maroldi, R, O'Sullivan, B, Paterson, C, Tagliaferri, L, Tribius, S, Yom, SS & Gregoire, V 2025, 'Delineation of the post-operative primary tumour and nodal clinical target volumes in oral cavity squamous cell carcinoma: European Society for Radiotherapy and Oncology (ESTRO) clinical guidelines', Radiotherapy and Oncology, vol. 212, 111135. https://doi.org/10.1016/j.radonc.2025.111135

Merriman, NA, Penfold, RS, Walsh, ME, Sexton, E, Brent, L, Hickey, P, Coughlan, T, Ojeda-Thies, C, Johansen, A, Hall, AJ, MacLullich, AMJ, O’Regan, N, Blake, C, National Collaborators, C, Fasig, B, Hommel, A, Queirós, C, Carvalho, B, Khan, AH, Chinoy, MA, Gjertsen, JE, van Bremen, HE, Duarte-Flores, JO, Yamamoto, N, Ferrara, MC, Bellelli, G, Sund, R, Iliopoulos, E, Schmidt, H, Höfer, C, Kristensen, PK , Pedersen, AB, Saguier, M, Monteverde, E, Diehl, M & Bosque, L 2025, 'Delirium and cognitive assessment in national hip fracture registries: a scoping review', European Geriatric Medicine, vol. 16, no. 5, pp. 1679-1701. https://doi.org/10.1007/s41999-025-01246-4

Displaying results 7901 to 7950 out of 391130

159

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen