Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Korsgaard, MB 2019, 'Audiovisual Literacy and the Ghost of Silent Cinema in Contemporary YouTube Clips', Quarterly Review of Film and Video, vol. 36, no. 7, pp. 573-583. https://doi.org/10.1080/10509208.2019.1593017

Christoffersen, EE 2019, 'Audition på Studioscenen', Peripeti. <http://www.peripeti.dk/2019/05/10/audition-paa-aarhus-teater/>

Hansen, V , Asmund, G & Birch Larsen, M 2019, Audit of Kvanefjeld REEs, U and Zn project: Exploration activities 2007-2017, 29 August to 5 September 2017 . Scientific Report from DCE - Danish Centre for Environment and Energy, no. 332, Aarhus University, DCE - Danish Centre for Environment and Energy.

Blenkmann, AO, Collavini, S, Lubell, J, Llorens, A, Funderud, I, Ivanovic, J, Larsson, PG, Meling, TR, Bekinschtein, T, Kochen, S, Endestad, T, Knight, RT & Solbakk, A-K 2019, 'Auditory deviance detection in the human insula: An intracranial EEG study', Cortex, vol. 121, pp. 189-200. https://doi.org/10.1016/j.cortex.2019.09.002

Robson, H, Griffiths, TD, Grube, M & Woollams, AM 2019, 'Auditory, Phonological, and Semantic Factors in the Recovery From Wernicke’s Aphasia Poststroke: Predictive Value and Implications for Rehabilitation', Neurorehabilitation and Neural Repair, vol. 33, no. 10, pp. 800-812. https://doi.org/10.1177/1545968319868709

Bauer, TJ, Gombocz, E, Krüger, M, Sahana, J , Corydon, TJ, Bauer, J, Infanger, M & Grimm, D 2019, 'Augmenting cancer cell proteomics with cellular images - a semantic approach to understand focal adhesion', Journal of Biomedical Informatics, vol. 100, 103320. https://doi.org/10.1016/j.jbi.2019.103320

Griggio, CF, Nouwens, M, McGrenere, J & Mackay, WE 2019, Augmenting couples' communication with lifelines: Shared timelines of mixed contextual information. in CHI 2019 - Proceedings of the 2019 CHI Conference on Human Factors in Computing Systems. Association for Computing Machinery, 2019 CHI Conference on Human Factors in Computing Systems, CHI 2019, Glasgow, United Kingdom, 04/05/2019. https://doi.org/10.1145/3290605.3300853

Jensen, PM & Jacobsen, UC 2019, 'Augmenting proximities theory: Including other proximities in the transnational travel of Danish television drama', Paper presented at NordMedia 2019, Malmö, Sweden, 21/08/2019 - 23/08/2019.

Sharma, K, Equbal, A , Nielsen, NC & Madhu, PK 2019, 'A unified heteronuclear decoupling picture in solid-state NMR under low radio-frequency amplitude and fast magic-angle-spinning frequency regime', Journal of Chemical Physics, vol. 150, no. 14, 144201. https://doi.org/10.1063/1.5082352

Shivayogimath, A, Thomsen, JD, Mackenzie, DMA, Geisler, M, Stan, RM , Holt, AJ , Bianchi, M, Crovetto, A, Whelan, PR, Carvalho, A, Neto, AHC, Hofmann, P, Stenger, N, Bøggild, P & Booth, TJ 2019, 'A universal approach for the synthesis of two-dimensional binary compounds', Nature Communications, vol. 10, no. 1, 2957. https://doi.org/10.1038/s41467-019-11075-2

Johnson, MG, Pokorny, L, Dodsworth, S, Botigué, LR, Cowan, RS, Devault, A, Eiserhardt, WL, Epitawalage, N, Forest, F, Kim, JT, Leebens-Mack, JH, Leitch, IJ, Maurin, O, Soltis, DE, Soltis, PS, Wong, GKS, Baker, WJ & Wickett, NJ 2019, 'A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-Medoids Clustering', Systematic Biology, vol. 68, no. 4, pp. 594-606. https://doi.org/10.1093/sysbio/syy086

Acharya, K , Beliatis, M & Presser, M 2019, AUR: A unit to relate: Regulating air conditioning use with IoT to address social disparity in urban Indian homes. in Global IoT Summit, GIoTS 2019 - Proceedings., 8766392, IEEE, 3rd Global IoT Summit, GIoTS 2019, Aarhus, Denmark, 17/06/2019. https://doi.org/10.1109/GIOTS.2019.8766366

Jensen, NR 2019, 'Austerity and the implementation of the Europe 2020 strategy in Spain. Re-shaping the European productive and social model: a reflexion from the south', European Journal of Social Work, vol. 22, no. 4, pp. 727-728. https://doi.org/10.1080/13691457.2019.1576315

Vohnsen, NH & Bech Knudsen, L 2019, 'Austerity as ‘state practice’: Poverty, imagination, and ‘the future’ in Scottish policy-making', Paper presented at Genealogies and Positionalities of Thinking the State, Leiden, Netherlands, 30/10/2019 - 01/11/2019.

Christoffersen, EE 2019, 'Autenticitet og immersion i SIGNAS Det Åbne Hjerte', Peripeti.

Prevéy, JS, Rixen, C, Rüger, N, Høye, TT , Bjorkman, AD, Myers-Smith, IH, Elmendorf, SC, Ashton, IW, Cannone, N, Chisholm, CL, Clark, K, Cooper, EJ, Elberling, B, Fosaa, AM, Henry, GHR, Hollister, RD, Jónsdóttir, IS, Klanderud, K, Kopp, CW, Lévesque, E, Mauritz, M, Molau, U, Natali, SM, Oberbauer, SF, Panchen, ZA, Post, E, Rumpf, SB, Schmidt, NM, Schuur, E, Semenchuk, PR, Smith, JG, Suding, KN, Totland, Ø, Troxler, T, Venn, S, Wahren, CH, Welker, JM & Wipf, S 2019, 'Author Correction: Warming shortens flowering seasons of tundra plant communities', Nature Ecology & Evolution, vol. 3, pp. 709-709. https://doi.org/10.1038/s41559-019-0859-5

Kaur, H, Carvalho, J, Looso, M, Singh, P, Chennupati, R, Preussner, J, Günther, S, Albarrán-Juárez, J, Tischner, D, Classen, S, Offermanns, S & Wettschureck, N 2019, 'Author Correction: Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system', Nature Communications, vol. 10, no. 1, 1448, pp. 1448. https://doi.org/10.1038/s41467-019-09334-3

Luyssaert, S, Marie, G, Valade, A, Chen, YY, Djomo, SN, Ryder, J, Otto, J, Naudts, K, Lansø, AS, Ghattas, J & McGrath, MJ 2019, 'Author Correction: Trade-offs in using European forests to meet climate objectives', Nature, vol. 567, no. 7748, pp. E13. https://doi.org/10.1038/s41586-019-1023-8

Natali, SM, Watts, JD, Rogers, BM, Potter, S, Ludwig, SM, Selbmann, AK, Sullivan, PF, Abbott, BW, Arndt, KA, Birch, L, Björkman, MP, Bloom, AA, Celis, G, Christensen, TR , Christiansen, CT, Commane, R, Cooper, EJ, Crill, P, Czimczik, C, Davydov, S, Du, J, Egan, JE, Elberling, B, Euskirchen, ES, Friborg, T, Genet, H, Göckede, M, Goodrich, JP, Grogan, P, Helbig, M, Jafarov, EE, Jastrow, JD, Kalhori, AAM, Kim, Y, Kimball, JS, Kutzbach, L, Lara, MJ, Larsen, KS, Lee, BY, Liu, Z, Loranty, MM, Lund, M, Lupascu, M, Madani, N, Malhotra, A, Matamala, R, McFarland, J, McGuire, AD, Michelsen, A, Minions, C, Oechel, WC, Olefeldt, D, Parmentier, FJW, Pirk, N, Poulter, B, Quinton, W, Rezanezhad, F, Risk, D, Sachs, T, Schaefer, K, Schmidt, NM, Schuur, EAG, Semenchuk, PR, Shaver, G, Sonnentag, O, Starr, G, Treat, CC, Waldrop, MP, Wang, Y, Welker, J, Wille, C, Xu, X, Zhang, Z, Zhuang, Q & Zona, D 2019, 'Author Correction: Large loss of CO₂ in winter observed across the northern permafrost region (Nature Climate Change, (2019), 9, 11, (852-857), 10.1038/s41558-019-0592-8)', Nature Climate Change, vol. 9, pp. 1005-1005. https://doi.org/10.1038/s41558-019-0644-0

Koefoed, NJ 2019, 'Authorities who care: The Lutheran Doctrine of the Three Estates in Danish Legal Development from the Reformation to Absolutism', Scandinavian Journal of History, vol. 44, no. 4, pp. 430-453. https://doi.org/10.1080/03468755.2018.1562965

Kirkegård, J & Mortensen, FV 2019, 'Author response to: It is time for a biological definition of locally advanced pancreatic cancer', British Journal of Surgery, vol. 106, no. 9, pp. 1257-1258. https://doi.org/10.1002/bjs.11250

Ranger, TA, Cicuttini, FM, Jensen, TS, Heritier, S & Urquhart, DM 2019, 'Author's response to letter to editor: “Confounding variables in future studies assessing relationship between paraspinal muscles and low back pain”', Spine Journal, vol. 19, no. 6, pp. 1134-1135. https://doi.org/10.1016/j.spinee.2019.02.008

Van Kessel, R, Walsh, S, Ruigrok, ANV, Holt, R, Yliherva, A, Kärnä, E, Moilanen, I, Hjörne, E, Johansson, ST, Schendel, D, Pedersen, L, Jørgensen, M, Brayne, C, Baron-Cohen, S & Roman-Urrestarazu, A 2019, 'Autism and the right to education in the EU: Policy mapping and scoping review of Nordic countries Denmark, Finland, and Sweden', Molecular Autism, vol. 10, no. 1, 44. https://doi.org/10.1186/s13229-019-0290-4

Satterstrom, FK, Walters, RK, Singh, T, Wigdor, EM, Lescai, F , Demontis, D, Kosmicki, JA, Grove, J, Stevens, C, Bybjerg-Grauholm, J, Bækvad-Hansen, M, Palmer, DS, Maller, JB, iPSYCH-Broad Consortium, Nordentoft, M, Mors, O, Robinson, EB, Hougaard, DM, Werge, TM, Bo Mortensen, P, Neale, BM, Børglum, AD & Daly, MJ 2019, 'Autism spectrum disorder and attention deficit hyperactivity disorder have a similar burden of rare protein-truncating variants', Nature Neuroscience, vol. 22, no. 12, pp. 1961-1965. https://doi.org/10.1038/s41593-019-0527-8

Long, M , Ghisari, M , Kjeldsen, LS , Wielsøe, M, Nørgaard-Pedersen, B, Erik lykke, M, Abdallah, M & Bonefeld-Jørgensen, EC 2019, 'Autism spectrum disorders, endocrine disrupting compounds and heavy metals in the amniotic fluid: A case-control study', Molecular Autism, vol. 10, no. 1, 1, pp. 1. https://doi.org/10.1186/s13229-018-0253-1

Gehrt, TB , Frostholm, L , Obermann, M-L & Berntsen, D 2019, 'Autobiographical memory and episodic future thinking in severe health anxiety: A comparison with obsessive-compulsive disorder', Journal of Psychosomatic Research, vol. 121, pp. 112-113. https://doi.org/10.1016/j.jpsychores.2019.03.043

Gehrt, TB 2019, 'Autobiographical memory in severe health anxiety', Aarhus. <https://soeg.kb.dk/permalink/45KBDK_KGL/1pioq0f/alma99123305014005763>

Zhou, Y, Zhou, L, Zhang, Y, Garcia de Souza, J, Podgorski, DC, Spencer, RGM, Jeppesen, E & Davidson, TA 2019, 'Autochthonous dissolved organic matter potentially fuels methane ebullition from experimental lakes', Water Research, vol. 166, 115048. https://doi.org/10.1016/j.watres.2019.115048

Valbjørn, M 2019, 'Autokratisk identitetspolitik i et 'sekteriseret' nyt Mellemøsten', Samfundsfagsnyt, no. 213, pp. 22-26.

Solheim, SA, Bejder, J, Breenfeldt Andersen, A, Mørkeberg, J & Nordsborg, NB 2019, 'Autologous blood transfusion enhances exercise performance - strength of the evidence and physiological mechanisms', Sports Medicine - Open, vol. 5, 30. https://doi.org/10.1186/s40798-019-0204-1

Holm, L , Stucke-Brander, T , Wagner, SR , Sandager, P , Schlütter, JM , Lindahl, C & Uldbjerg, N 2019, 'Automated blood pressure self-measurement station compared to office blood pressure measurement for first trimester screening of pre-eclampsia', Health Informatics Journal, vol. 25, no. 4, pp. 1815-1824. https://doi.org/10.1177/1460458218799505

Nielbo, KL, Perner, ML, Larsen, C, Nielsen, J & Laursen, D 2019, 'Automated compositional change detection in Saxo Grammaticus’ Gesta Danorum', CEUR Workshop Proceedings, vol. 2364, pp. 321-332.

Stærk, M 2019, 'Automated External Defibrillators are Rarely Used for In-Hospital Cardiac Arrest in Denmark', Resuscitation Science Symposium 2019, Philadelphia, United States, 15/11/2019 - 17/11/2019.

Stærk, M , Glerup Lauridsen, K , Krogh, K , Kirkegaard, H & Løfgren, B 2019, 'Automated External Defibrillators Are Rarely Used for In-Hospital Cardiac Arrest in Denmark', Circulation, vol. 140, no. Suppl_2, A109. https://doi.org/10.1161/circ.140.suppl_2.109

Nardello, M, Han, S, Møller, C & Gøtze, J 2019, 'Automated Modeling with Abstraction for Enterprise Architecture (AMA4EA): Business Process Model Automation in an Industry 4.0 Laboratory', Complex Systems Informatics and Modeling Quarterly, vol. 2019, no. 19, 111, pp. 42-59. https://doi.org/10.7250/csimq.2019-19.03

Juul, MØØ , Olsen, P & Brincker, R 2019, 'Automated OMA using shortest path detection', Ikke-valideret tidsskrift.

Olsen, P , Juul, MØØ , Tarpø, MG & Brincker, R 2019, 'Automated Operational Modal Analysis using Sliding Filters and Time Domain Identification Techniques', Mechanical Systems and Signal Processing.

Tarpø, M, Friis, T, Olsen, P, Juul, M, Georgakis, C & Brincker, R 2019, 'Automated reduction of statistical errors in the estimated correlation function matrix for operational modal analysis', Mechanical Systems and Signal Processing, vol. 132, pp. 790-805. https://doi.org/10.1016/j.ymssp.2019.07.024

Kristensen, EK 2019, 'Automated Techniques for Creation and Maintenance of TypeScript Declaration Files'.

Zhang, Y, Zhang, S, Westra, J, Ding, D, Zhao, Q, Yang, J, Sun, Z, Huang, J, Pu, J, Xu, B & Tu, S 2019, 'Automatic coronary blood flow computation: validation in quantitative flow ratio from coronary angiography', The International Journal of Cardiovascular Imaging, vol. 35, no. 4, pp. 587-595. https://doi.org/10.1007/s10554-018-1506-y

Ren, J, Arash Moaddel, H, Hauge, EM , Keller, KK , Jensen, RK & Lauze, F 2019, Automatic detection and localization of bone erosion in hand HR-pQCT. in K Mori & HK Hahn (eds), Medical Imaging 2019: Computer-Aided Diagnosis., 1095022, SPIE - International Society for Optical Engineering, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10950, Medical Imaging 2019: Computer-Aided Diagnosis, San Diego, United States, 17/02/2019. https://doi.org/10.1117/12.2512876

Ärje, J, Milioris, D, Tran, DT, Jepsen, JU, Raitoharju, J, Gabbouj, M, Iosifidis, A & Høye, TT 2019, Automatic Flower Detection and Classification System Uing a Light-Weight Convolutional Neural Network. in 27th European Signal Processing Conference EUSIPCO 2019. IEEE, Proceedings of the European Signal Processing Conference, European Signal Processing Conference 2019, La Coruna, Spain, 02/09/2019. <http://eusipco2019.org/Proceedings/papertitles.html>

Kragh, MF, Rimestad, J, Berntsen, J & Karstoft, H 2019, 'Automatic grading of human blastocysts from time-lapse imaging', Computers in Biology and Medicine, vol. 115, 103494. https://doi.org/10.1016/j.compbiomed.2019.103494

Kragh, MF, Rimestad, J, Berntsen, J & Karstoft, H 2019, 'Automatic morphological grading of human blastocysts with time-lapse imaging and artificial intelligence', ESHRE, Vienna, Austria, 23/06/2019 - 26/06/2019. <https://www.eshre.eu/ESHRE2019/Programme/Searchable#!abstractdetails/0000573820>

Andersen, KJA 2019, 'Automatic Program Verification'.

Gangstad, SW, Mikkelsen, KB , Kidmose, P , Tabar, YR, Weisdorf, S, Lauritzen, MH, Hemmsen, MC, Hansen, LK, Kjaer, TW & Duun-Henriksen, J 2019, 'Automatic sleep stage classification based on subcutaneous EEG in patients with epilepsy', BioMedical Engineering Online, vol. 18, no. 1, 106. https://doi.org/10.1186/s12938-019-0725-3

Benetton, JB, Neave, H, Costa, JHC, von Keyserlingk, MAG & Weary, DM 2019, 'Automatic weaning based on individual solid feed intake: Effects on behavior and performance of dairy calves', Journal of Dairy Science, vol. 102, no. 6, pp. 5475-5491. https://doi.org/10.3168/jds.2018-15830

Istrup, P 2019, 'Automatiseret sagsbehandling og retssikkerhed. Venner eller fjender?', Advokaten, vol. 2019, no. 4, Advokaten 4 - 03-05-2019, pp. 40-42. <https://www.advokatsamfundet.dk/Service/Publikationer/Tidligere%20artikler/2019/Advokaten%204/Automatiseret%20sagsbehandling%20og%20retssikkerhed%20Venner%20eller%20fjender.aspx>

Bager-Elsborg, A & Herrmann, KJ 2019, 'Automatized online study skill counselling. Will students appreciate the advice?', EdMedia + Innovate Learning, Amsterdam, Netherlands, 24/06/2019 - 28/06/2019.

Larsen, P 2019, 'Automatrifler, cannabisplanter og frisk kokain: Det tyske rapfænomen Gzuz er i virkeligheden et dannet bæst', Dagbladet Information.

Displaying results 94551 to 94600 out of 390616

1892

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen