Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Hermansen, C , Knadel, M, Moldrup, P, Greve, MH , Gislum, R & de Jonge, LW 2015, 'Prediction of mineral fines to soil organic carbon ratio with visible near-infrared spectroscopy (vis-NIRS)', ASA, CSSA and SSSA International Annual Meeting 2015, Minneapolis, United States, 15/11/2015 - 18/11/2015.

Hermansen, C , Knadel, M, Moldrup, P, Greve, MH , Karup, D & de Jonge, LW 2015, 'Prediction of particle size distribution with visible near-infrared spectroscopy', ASA, CSSA and SSSA International Annual Meeting 2015, Minneapolis, United States, 15/11/2015 - 18/11/2015.

Nielsen, TK & Hansen, LH 2015, 'Prediction of plasmid contigs in sphingomonad genomes', Dansk Mikrobiologisk Selskab, Copenhagen, Denmark, 09/11/2015 - 09/11/2015.

Choi, D, Fox, Z, Albert, T, Arts, M, Balabaud, L, Bunger, C, Buchowski, JM, Coppes, MH, Depreitere, B, Fehlings, MG, Harrop, J, Kawahara, N, Martin-Benlloch, JA, Massicotte, EM, Mazel, C, Oner, FC, Peul, W, Quraishi, N, Tokuhashi, Y, Tomita, K, Verlaan, JJ, Wang, M & Crockard, HA 2015, 'Prediction of Quality of Life and Survival After Surgery for Symptomatic Spinal Metastases: A Multicenter Cohort Study to Determine Suitability for Surgical Treatment', Neurosurgery. https://doi.org/10.1227/NEU.0000000000000907

Gregersen, M, Brink, O, Krogshede, A & Damsgaard, EMS 2015, 'Prediction of Reoperation of Femoral Neck Fractures Treated With Cannulated Screws in Elderly Patients', Geriatric Orthopaedic Surgery & Rehabilitation, vol. 6, no. 4, pp. 322-327. https://doi.org/10.1177/2151458515614369

Parad, RB, Davis, JM, Lo, J, Thomas, M, Marlow, N, Calvert, S, Peacock, JL & Greenough, A 2015, 'Prediction of respiratory outcome in extremely low gestational age infants', Neonatology, vol. 107, no. 4, pp. 241-248. https://doi.org/10.1159/000369878

Foskolos, A, Calsamiglia, S, Chrenková, M, Weisbjerg, MR & Albanell, E 2015, 'Prediction of rumen degradability parameters of a wide range of forages and non-forages by NIRS', Animal, vol. 9, no. 7, pp. 1163-1171. https://doi.org/10.1017/S1751731115000191

Paradelo Pérez, M , Norgaard, T, Moldrup, P, Ferrè, TPA, Kahawaththa Gamage, IDK , Arthur, E & de Jonge, LW 2015, 'Prediction of the glyphosate sorption coefficient across two loamy agricultural fields', Geoderma, vol. 259-260, pp. 224-232. https://doi.org/10.1016/j.geoderma.2015.06.011

Alibrandi, U, Ma, C, Low, YM & Koh, CG 2015, Prediction of the response of a SCR using the Multi-Gaussian Maximum Entropy Method. in ASME 2015 34th International Conference on Ocean, Offshore and Arctic Engineering. vol. 3: Structures, Safety and Reliability, OMAE2015-41221, American Society of Mechanical Engineers (ASME), 34th International Conference on Ocean, Offshore and Arctic Engineering, St. Johns, Newfoundland and Labrador, Canada, 31/05/2015. https://doi.org/10.1115/OMAE2015-41221

Vibart, RE, Vogeler, I, Cichota, R & Horne, D 2015, Predictions of nitrogen leaching from a well-drained soil under dryland and irrigated dairy farming using APSIM and OVERSEER. in T Weber, M McPhee & R Anderssen (eds), Proceedings - 21st International Congress on Modelling and Simulation, MODSIM 2015. Modelling and Simulation Society of Australia and New Zealand Inc. (MSSANZ), pp. 406-412, 21st International Congress on Modelling and Simulation: Partnering with Industry and the Community for Innovation and Impact through Modelling, MODSIM 2015 - Held jointly with the 23rd National Conference of the Australian Society for Operations Research and the DSTO led Defence Operations Research Symposium, DORS 2015, Broadbeach, Australia, 29/11/2015.

Gebauer, L , Kringelbach, ML & Vuust, P 2015, 'Predictive coding links perception, action, and learning to emotions in music. Comment on "The quartet theory of human emotions: An integrative and neurofunctional model" by S. Koelsch et al.', Physics of Life Reviews, vol. 13, pp. 50-52. https://doi.org/10.1016/j.plrev.2015.04.023

Soares, A , Paradelo Pérez, M, Møldrup, P, Delerue-Matos, C & de Jonge, LW 2015, 'Predictivity strength of the spatial variability of phenanthrene sorption across two sandy loam fields', Water, Air and Soil Pollution, vol. 226, 36. https://doi.org/10.1007/s11270-015-2305-x

Kyhl, K, Vejlstrup, N, Lønborg, J, Treiman, M, Ahtarovski, KA, Helqvist, S, Kelbæk, H, Holmvang, L, Jørgensen, E, Saunamäki, K, Søholm, H, Andersen, MJ , Møller, JE, Clemmensen, P & Engstrøm, T 2015, 'Predictors and prognostic value of left atrial remodelling after acute myocardial infarction', Open Heart, vol. 2, no. 1, pp. e000223. https://doi.org/10.1136/openhrt-2014-000223

Torp, NC, Dahl, K, Skarphedinsson, G, Compton, S, Thomsen, PH, Weidle, B, Hybel, KA, Valderhaug, R, Melin, K, Nissen, JB & Ivarsson, T 2015, 'Predictors associated with improved cognitive-behavioral therapy outcome in pediatric obsessive-compulsive disorder', Journal of the American Academy of Child and Adolescent Psychiatry, vol. 54, no. 3, pp. 200-207. https://doi.org/10.1016/j.jaac.2014.12.007

Granild-Jensen, JB , Rackauskaite, G, Flachs, EM & Uldall, P 2015, 'Predictors for early diagnosis of cerebral palsy from national registry data', Developmental Medicine and Child Neurology, vol. 57, no. 10, pp. 931-935. https://doi.org/10.1111/dmcn.12760

Aagaard, J & Kølbæk, P 2015, 'Predictors of Clinical Outcome of Assertive Community Treatment (ACT) in a Rural Area in Denmark: An Observational Study with a Two-Year Follow-Up', Community Mental Health Journal. https://doi.org/10.1007/s10597-015-9908-y

Møller, PK, Tolstrup, JS, Olsen, MH, Dalton, SO, Overgaard, J & Johansen, J 2015, 'Predictors of continuous tobacco smoking in a clinical cohort study of Danish laryngeal cancer patients smoking before treated with radiotherapy', Acta Oncologica, vol. 54, no. 5, pp. 685-92. https://doi.org/10.3109/0284186X.2014.996665

Kotzé, SR, Pedersen, OB, Petersen, MS, Sørensen, E, Thørner, LW, Sørensen, CJ, Rigas, AS, Hjalgrim, H, Rostgaard, K, Ullum, H & Erikstrup, C 2015, 'Predictors of hemoglobin in Danish blood donors: results from the Danish Blood Donor Study', Transfusion, vol. 55, no. 6, pp. 1303-11. https://doi.org/10.1111/trf.13011

El Ansari, W, Vallentin-Holbech, L & Stock, C 2015, 'Predictors of illicit drug/s use among university students in Northern Ireland, Wales and England', Global Journal of Health Science, vol. 7, no. 4, pp. 18-29. https://doi.org/10.5539/gjhs.v7n4p18

Pedersen, MKJ , Mackenhauer, J , Hvass, AMSW , Heide-Jørgensen, U , Christiansen, CF & Kirkegaard, H 2015, 'Predictors of intensive care unit transfer or death in emergency department patients with suspected infection', European Journal of Emergency Medicine, vol. 22, no. 3, pp. 176-180. https://doi.org/10.1097/MEJ.0000000000000200

Ozer, S 2015, 'Predictors of international students’ psychological and sociocultural adjustment to the context of reception while studying at Aarhus University, Denmark', Scandinavian Journal of Psychology, vol. 56, no. 6, pp. 717-725. https://doi.org/10.1111/sjop.12258

Lasgaard, M & Friis, K 2015, 'Predictors of loneliness across the age-span', 8TH EUROPEAN PUBLIC HEALTH CONFERENCE, Milan, Italy, 14/10/2015 - 17/10/2015.

Lasgaard, MK & Friis, K 2015, 'Predictors of loneliness across the age-span: Mathias Lasgaard', European Journal of Public Health, vol. 25, no. Supp 3, pp. 450. https://doi.org/10.1093/eurpub/ckv176.204

Ozer, S 2015, 'Predictors of mental health amongst Ladakhi college students', Ladakh Studies, vol. 33, pp. 4-17.

Nierenberg, AA, Østergaard, SD, Iovieno, N, Walker, RSW, Fava, M & Papakostas, GI 2015, 'Predictors of placebo response in bipolar depression', International Clinical Psychopharmacology, vol. 30, no. 2, pp. 59-66. https://doi.org/10.1097/YIC.0000000000000058

Vase, L, Vollert, J, Finnerup, NB, Miao, X, Atkinson, G, Marshall, S, Nemeth, R, Lange, B, Liss, C, Price, DD, Maier, C, Jensen, TS & Segerdahl, M 2015, 'Predictors of the placebo analgesia response in randomized controlled trials of chronic pain: a meta-analysis of the individual data from nine industrially sponsored trials', Pain, vol. 156, no. 9, pp. 1795-802. https://doi.org/10.1097/j.pain.0000000000000217

Gasse, C , Wimberley, T , Støvring, H, Sørensen, HJ & MacCabe, JH 2015, 'PREDICTORS OF TREATMENT RESISTANCE IN SCHIZOPHRENIA', Schizophrenia Bulletin, vol. 41, no. Suppl 1, pp. S138-S139. https://doi.org/10.1093/schbul/sbv010

Schmidt, H, Von Vangerow, J, Stienkemeier, F, Bogomolov, AS, Baklanov, AV, Reich, DM, Skomorowski, W, Koch, CP & Mudrich, M 2015, 'Predissociation dynamics of lithium iodide', Journal of Chemical Physics, vol. 142, no. 4, 044303. https://doi.org/10.1063/1.4906512

Ruperto, N, Pistorio, A, Oliveira, S, Zulian, F, Cuttica, R, Ravelli, A, Fischbach, M, Magnusson, B, Sterba, G, Avcin, T, Brochard, K, Corona, F, Dressler, F, Gerloni, V, Apaz, MT, Bracaglia, C, Cespedes-Cruz, A, Cimaz, R, Couillault, G, Joos, R, Quartier, P, Russo, R, Tardieu, M, Wulffraat, N, Bica, B, Dolezalova, P, Ferriani, V, Flato, B, Bernard-Medina, AG, Herlin, T, Trachana, M, Meini, A, Allain-Launay, E, Pilkington, C, Vargova, V, Wouters, C, Angioloni, S, Martini, A & Paediatric Rheumatology International Trials Organisation (PRINTO) 2015, 'Prednisone versus prednisone plus ciclosporin versus prednisone plus methotrexate in new-onset juvenile dermatomyositis: a randomised trial', The Lancet, vol. 387, no. 10019, pp. 671-678. https://doi.org/10.1016/S0140-6736(15)01021-1

Bergink, V , Laursen, TM , Johannsen, BMW, Kushner, SA, Meltzer-Brody, S & Munk-Olsen, T 2015, 'Pre-eclampsia and first-onset postpartum psychiatric episodes: a Danish population-based cohort study', Psychological Medicine, vol. 45, no. 16, pp. 3481-3489. https://doi.org/10.1017/S0033291715001385

Christensen, M, Kronborg, CS & Knudsen, UB 2015, 'Preeclampsia and the postpartum cardiovascular risk – a 10-year follow-up.', PhD Day, Aarhus, Denmark, 30/01/2015.

Gamst, J , Christiansen, CF , Rasmussen, BS , Rasmussen, LH & Thomsen, RW 2015, 'Pre-existing atrial fibrillation and risk of arterial thromboembolism and death in intensive care unit patients: a population-based cohort study', Critical Care, vol. 19, no. 1, pp. 299. https://doi.org/10.1186/s13054-015-1007-5

Bakken Jepsen, J, De Clerck, G, Lutalo-Kiingi, S & Mcgregor, W 2015, Preface. in J Bakken Jepsen, G De Clerck, S Lutalo-Kiingi & W B. McGregor (eds), Sign languages of the World: A Comparative Handbook. 1 edn, De Gruyter, Berlin, pp. v-vi.

Larsen, KB 2015, Preface. in KB Larsen (ed.), The Gospel of John as Genre Mosaic. Vandenhoeck & Ruprecht, Göttingen, Studia Aarhusiana Neotestamentica, vol. 3, pp. 11.

Mortensen, E & Saxkjær, SG 2015, Preface. in E Mortensen & SG Saxkjær (eds), Revealing and Concealing in Antiquity: Textual and Archaeological Approaches to Secrecy. Aarhus Universitetsforlag, Aarhus, Aarhus Studies in Mediterranean Antiquity, vol. XIII, pp. 7-9.

Kemp, P 2015, Preface. in P Kemp & N Hashimoto (eds), Ethics and Politics : ethique et politique: with a third part on Paul Ricœur. LIT Verlag, Zürich, Eco-ethica, vol. 4, pp. 7.

Grøn, CH, Nedergaard, P & Wivel, A 2015, Preface. in The Nordic Countries and the European Union: Still the Other European Community?. Taylor and Francis Inc., pp. xx-xxi. https://doi.org/10.4324/9781315726335

Gunnarsson, E, Hansen, HP, Nielsen, BS & Sriskandarajah, N 2015, Preface. in Action Research for Democracy: New Ideas and Perspectives from Scandinavia. Taylor and Francis Press, pp. ix-x. https://doi.org/10.4324/9781315659909

Kreiser, L, Andersen, MS , Olsen, BE, Speck, S, Milne, JE & Ashiabor, H 2015, Preface. in Environmental Pricing: Studies in Policy Choices and Interactions. vol. 16, Edward Elgar Publishing, pp. xv. https://doi.org/10.4337/9781785360251

Kreiser, L, Andersen, MS , Olsen, BE, Speck, S, Milne, JE & Ashiabor, H 2015, Preface. in Carbon Pricing: Design, Experiences and Issues. vol. 15, Edward Elgar Publishing, pp. xiv. https://doi.org/10.4337/9781785360237

Bruun, J 2015, Preface and Introduction. in J Bruun & J Lieberkind (eds), Challenges of Citizenship Education: a Danish Case Study. 1 edn, U Press, København, pp. 7-11. <http://upress.dk/boger/challenges-of-citizenship-education/>

Stadler, B 2015, 'Preface to Special Topic: Microfluidics in Drug Delivery', Biomicrofluidics, vol. 9, no. 5, 052501. https://doi.org/10.1063/1.4931070

Shrestha, G , Skovgård, H , Steenberg, T & Enkegaard, A 2015, 'Preference and life history traits of Aphelinus abdominalis (Hymenoptera: Aphelinidae) when offered different development stages of the lettuce aphid Nasonovia ribisnigri (Hemiptera: Aphididae)', BioControl, vol. 60, no. 4, pp. 463-471. https://doi.org/10.1007/s10526-015-9661-8

Jensen, MB , Herskin, MS , Thomsen, PT, Forkman, B & Houe, H 2015, 'Preferences of lame cows for type of surface and level of social contact in hospital pens', Journal of Dairy Science, vol. 98, no. 7, pp. 4552-4559. https://doi.org/10.3168/jds.2014-9203

Afesorgbor, SK & Abreha, KG 2015 'Preferential Market Access, Foreign Aid and Economic Development' Economics Working Papers, no. 2015-04, Institut for Økonomi, Aarhus Universitet, Aarhus.

Han, E, Kautz, T, Huang, N & Köpke, U 2015, 'Preferential root growth through biopore channels increases rooting density of barley and canola in the subsoil', 9th ISRR sympositum, Canberra, Australia, 01/10/2015.

Povlsen, BB, Da Aw, L, Laursen, RJ , Esteves, SC & Humaidan, P 2015, 'Pregnancy and birth after intracytoplasmic sperm injection with normal testicular spermatozoa in a patient with azoospermia and tail stump epididymal sperm', International braz j urol : official journal of the Brazilian Society of Urology, vol. 41, no. 6, pp. 1220-1225. https://doi.org/10.1590/S1677-5538.IBJU.2015.0296

Fuglsang, J & Ovesen, PG 2015, 'Pregnancy and delivery in a woman with type 1 diabetes, gastroparesis, and a gastric neurostimulator', Diabetes Care, vol. 38, no. 5, pp. e75. https://doi.org/10.2337/dc14-2959

Bøtkjær, JA, Jeppesen, JV, Wissing, ML, Kløverpris, S , Oxvig, C, Mason, JI, Borgbo, T & Andersen, CY 2015, 'Pregnancy-associated plasma protein A in human ovarian follicles and its association with intrafollicular hormone levels', Fertility and Sterility, vol. 104, no. 5, pp. 1294-1301. https://doi.org/10.1016/j.fertnstert.2015.07.1152

Mittal, A, Pachter, L, Nelson, JL, Kjærgaard, H, Smed, MK, Gildengorin, VL, Zoffmann, V, Hetland, ML, Jewell, NP, Olsen, J & Jawaheer, D 2015, 'Pregnancy-Induced Changes in Systemic Gene Expression among Healthy Women and Women with Rheumatoid Arthritis', PLoS One, vol. 10, no. 12, e0145204. https://doi.org/10.1371/journal.pone.0145204

Displaying results 165751 to 165800 out of 389757

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Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen