Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Broersen, LHA, Pereira, AM, Jørgensen, JOL & Dekkers, OM 2015, 'Response to the Letter by Lindholm, et al', Endocrinology, vol. 100, no. 8, pp. L66-7. https://doi.org/10.1210/JC.2015-2827

Liew, Z , Olsen, J, Cui, X, Ritz, B & Arah, OA 2015, 'Response to Werler and Parker letter: Comment on live-birth bias in pregnancy cohorts', International Journal of Epidemiology, vol. 44, no. 3, pp. 1080-1. https://doi.org/10.1093/ije/dyv140

Davies, SR & Horst, M 2015, Responsible Innovation in the US, UK and Denmark: Governance Landscapes. in B-J Koops, I Oosterlaken, H Romijn, T Swierstra & J van den Hoven (eds), Responsible Innovation 2: Concepts, Approaches, and Applications. Springer, pp. 37-56. https://doi.org/10.1007/978-3-319-17308-5_3

Christiansen, DH , Frost, P, Falla, D, Haahr, JP, Frich, LH & Svendsen, SW 2015, 'Responsiveness and minimal clinically important change: a comparison between two shoulder outcome measures', Journal of Orthopaedic and Sports Physical Therapy, vol. 45, no. 8, pp. 620-625. https://doi.org/10.2519/jospt.2015.5760

Horst, CE & Tireli, Ü 2015, Ressourcebegrebet i en interkulturel pædagogisk sammenhæng. in T Ritchie (ed.), Ressourcer i teori og praksis: en brugsbog for pædagoger. 1. edn, Billesøe og Baltzer, Værløse, pp. 195-214.

Pedersen, LH , Wogensen, L, Marcussen, N, Cecchi, CR , Dalsgaard, T & Dagnæs-Hansen, F 2015, 'Restoration of Haemoglobin Level Using Hydrodynamic Gene Therapy with Erythropoietin Does Not Alleviate the Disease Progression in an Anaemic Mouse Model for TGFβ1-Induced Chronic Kidney Disease', PLoS One, vol. 10, no. 6. https://doi.org/10.1371/journal.pone.0128367

Jensen, MM 2015, 'Restricted Cartan Type Lie Algebras and the Coadjoint Representation', Transformation Groups, vol. 20, no. 2, pp. 495-505. https://doi.org/10.1007/s00031-014-9295-4

Möllers, J & Oshima, Y 2015, 'Restriction of most degenerate representations of O(1,N) with respect to symmetric pairs', J. Math. Sci. Univ. Tokyo, vol. 22, no. 1, pp. 279-338.

Mastretta-Yanes, A, Arrigo, N, Alvarez, N, Jorgensen, TH, Piñero, D & Emerson, BC 2015, 'Restriction site-associated DNA sequencing, genotyping error estimation and de novo assembly optimization for population genetic inference', Molecular Ecology Resources, vol. 15, no. 1, pp. 28-41. https://doi.org/10.1111/1755-0998.12291

Gurjar, S 2015, 'Restriction Theorem for Principal bundles in Arbitrary Characteristic', Journal of Algebra, vol. 426, pp. 79-91. https://doi.org/10.1016/j.jalgebra.2014.10.051

Andersen, LW 2015, 'Resultater af DNA-analyser udført på indsendte spytprøver fra nedlagte husdyr fra 1. kvartal 2015 samt fra en individanalyse fra 2014', 4 p..

Andersen, LW 2015, 'Resultater af DNA-analyser udført på indsendte spytprøver fra nedlagte husdyr fra 2. og 3. kvartal 2015', 6 p..

Grøn, CH 2015, 'Resultater ved fronten – brugen af effektstyring i dagtilbud', Politik, vol. 18, no. 1.

Erdem, H, Cag, Y, Ozturk-Engin, D, Defres, S, Kaya, S, Larsen, L, Poljak, M, Barsic, B, Argemi, X, Sørensen, SM, Bohr, AL, Tattevin, P, Damsgaard Gunst, J, Baštáková, L, Jereb, M, Johansen, IS, Karabay, O, Pekok, AU, Sipahi, OR, Chehri, M, Beraud, G, Shehata, G, Del Vecchio, RF, Maresca, M, Karsen, H, Sengoz, G, Sunbul, M, Yilmaz, G, Yilmaz, H, Sharif-Yakan, A, Kanj, SS, Parlak, E, Pehlivanoglu, F, Korkmaz, F, Komur, S, Kose, S, Ulug, M, Bolukcu, S, Coskuner, SA, Ince, N, Akkoyunlu, Y, Halac, G, Sahin-Horasan, E, Tireli, H, Kilicoglu, G, Al-Mahdawi, A, Nemli, SA, Inan, A, Senbayrak, S, Stahl, JP & Vahaboglu, H 2015, 'Results of a multinational study suggest the need for rapid diagnosis and early antiviral treatment at the onset of herpetic meningoencephalitis', Antimicrobial Agents and Chemotherapy, vol. 59, no. 6, pp. 3084-9. https://doi.org/10.1128/AAC.05016-14

Hartvig, P , Jespersen, H , Lindberg, V , Sørensen, S , Madsen, L , Sørensen, J & Zielinski, M 2015, Results of the testing of herbicides, growth regulators and desiccants in agricultural crops and herbicides in horticultural crops 2014. in LN Jørgensen, BJ Nielsen, PK Jensen, P Kudsk, SK Mathiassen, P Hartvig & M Kristensen (eds), Applied Crop Protection 2014. DCA - Nationalt Center for Fødevarer og Jordbrug, DCA rapport, no. 058, pp. 138-140. <http://web.agrsci.dk/djfpublikation/index.asp?action=show&id=1197>

Winther-Jensen, M, Kjaergaard, J, Hassager, C, Bro-Jeppesen, J, Nielsen, N, Lippert, FK, Køber, L, Wanscher, M & Søholm, H 2015, 'Resuscitation and post resuscitation care of the very old after out-of-hospital cardiac arrest is worthwhile', International Journal of Cardiology, vol. 201, pp. 616-623. https://doi.org/10.1016/j.ijcard.2015.08.143

Kjær, TN , Thorsen, K , Jessen, N , Stenderup, K & Pedersen, SB 2015, 'Resveratrol ameliorates imiquimod-induced psoriasis-like skin inflammation in mice', PLoS One, vol. 10, no. 5, e0126599. https://doi.org/10.1371/journal.pone.0126599

Aschemann-Witzel, J & Grunert, KG 2015, 'Resveratrol and health from a consumer perspective: perception, attitude, and adoption of a new functional ingredient', Annals of the New York Academy of Sciences, vol. 1348, no. 1, pp. 171-179. https://doi.org/10.1111/nyas.12867

Poulsen, MM , Fjeldborg, K , Ornstrup, MJ , Kjær, TN , Nøhr, MK & Pedersen, SB 2015, 'Resveratrol and inflammation: Challenges in translating pre-clinical findings to improved patient outcomes', B B A - Molecular Basis of Disease, pp. 1124-1136. https://doi.org/10.1016/j.bbadis.2014.12.024

Aschemann-Witzel, J & Grunert, KG 2015, 'Resveratrol food supplements: a survey on the role of individual consumer characteristics in predicting the attitudes and adoption intentions of US American and Danish respondents', B M C Public Health, vol. 15, no. 110. https://doi.org/10.1186/s12889-015-1348-7

Kjaer, TN , Ornstrup, MJ , Poulsen, MM , Jørgensen, JOL, Hougaard, DM, Cohen, AS, Neghabat, S, Richelsen, B & Pedersen, SB 2015, 'Resveratrol reduces the levels of circulating androgen precursors but has no effect on, testosterone, dihydrotestosterone, PSA levels or prostate volume. A 4-month randomised trial in middle-aged men', Prostate, vol. 75, no. 12, pp. 1255-63. https://doi.org/10.1002/pros.23006

Sun, C, Walker, KL, Wakefield, DL & Dichtel, WR 2015, 'Retaining the Activity of Enzymes and Fluorophores Attached to Graphene Oxide', Chemistry of Materials, vol. 27, no. 12, pp. 4499-4504. https://doi.org/10.1021/acs.chemmater.5b01954

Normann, J & Bjerregaard, T 2015, Retention of Highly Skilled Expatriates and the Danish Work–Life Balance. in PH Andersen , H Kragh, JL & MS Linneberg (eds), Preparing for the Unexpected: Design of the Future Global Enterprise. Djøf Forlag, pp. 95-118.

Nakken, KO, Lindstrøm, P & Andersen, H 2015, 'Retention rate of zonisamide in intractable epilepsy', Acta Neurologica Scandinavica, vol. 131, no. 5, pp. 268-274. https://doi.org/10.1111/ane.12379

Odebrecht, C, Abreu, PC & Carstensen, J 2015, 'Retention time generates short-term phytoplankton blooms in a shallow microtidal subtropical estuary', Estuarine, Coastal and Shelf Science, vol. 162, pp. 35-44. https://doi.org/10.1016/j.ecss.2015.03.004

Moutsios, S 2015, 'Rethinking educational comparison under the conditions of globalisation', Paper presented at Comparative and International Education Society, Washington D.C., United States, 08/03/2015 - 13/03/2015.

Esbjerg, L 2015, 'Rethinking how retail buyers make buying decisions: From rational decision-making to retailer buying as relationally-responsive interaction', Paper presented at AMA/ACRA Second Triennual Conference, Coral Gables, United States, 04/03/2015 - 07/03/2015.

Eriksson, B & Stephensen, JL 2015, 'Rethinking Participation and Re-enacting Its Dilemmas? Aarhus 2017 and “The Playful Society”', Conjunctions : transdisciplinary journal of cultural participation, vol. 2, no. 2, pp. 48-66. https://doi.org/10.7146/tjcp.v2i2.22918

Larsen, CS 2015, 'Re-thinking prevention and treatment of travelers' diarrhea - Time for a change?', Travel Medicine and Infectious Disease, vol. 13, no. 3, pp. 211-2. https://doi.org/10.1016/j.tmaid.2015.05.006

Svejvig, P & Andersen, P 2015, 'Rethinking project management: A structured literature review with a critical look at the brave new world', International Journal of Project Management, vol. 33, no. 2, pp. 278-290. https://doi.org/10.1016/j.ijproman.2014.06.004

Lystbæk, CT 2015, 'Re-thinking reflection in supervision: A socio-cultural perspective on supervision in professional education', NERA 2015. 43th Annual Congress, Göteborg, Sweden, 04/03/2015 - 06/03/2015.

Nielsen, LP & Risgaard-Petersen, N 2015, 'Rethinking Sediment Biogeochemistry After the Discovery of Electric Currents', Annual Review of Marine Science, vol. 7, no. 1, pp. 425-442. https://doi.org/10.1146/annurev-marine-010814-015708

Deco, G, Tononi, G, Boly, M & Kringelbach, ML 2015, 'Rethinking segregation and integration: contributions of whole-brain modelling', Nature Reviews. Neuroscience, vol. 16, no. 7, pp. 430-9. https://doi.org/10.1038/nrn3963

Gupta, ND & Christensen, BJ 2015, 'Retirement and Health in the Nordic Welfare State', Nordic Economic Policy Review, vol. 2015, no. 2, pp. 173-195. <http://norden.diva-portal.org/smash/record.jsf?pid=diva2%3A901771&dswid=2008>

Vium, C & Taïx, C 2015, 'Retour au Bush', Le Monde, vol. Le Magasin du Monde, no. 216, pp. 66-75.

Mortensen, L , Berntsen, D & Bohn, O-S 2015, 'Retrieval of bilingual autobiographical memories: Effects of cue language and cue imageability', Memory, vol. 23, no. 2, pp. 138-156. https://doi.org/10.1080/09658211.2013.873809

Dubois, M-C, Bisegna, F, Gentile, N, Knoop, M, Matusiak, B, Osterhaus, W & Tetri, E 2015, 'Retrofitting the electric lighting and daylighting systems to reduce energy use in buildings: a literature review', Energy Research Journal, vol. 6, no. 1, pp. 25-41 . https://doi.org/10.3844/erjsp.2015.25.41

Kjærgaard, B , Hønge, JL, Magnusdottir, SO, Rasmussen, BS, Baandrup, U, Hasenkam, JM & Kristensen, SR 2015, 'Retrograde lung perfusion in the treatment of massive pulmonary embolism. A randomised porcine study', The 25th Annual Meeting of the Scandinavian Society for Research in Cardiothoracic Surgery, Geilo, Norway, 12/02/2015 - 14/02/2015.

Galassi, AR, Sianos, G, Werner, GS, Escaned, J, Tomasello, SD, Boukhris, M, Castaing, M, Büttner, JH, Bufe, A, Kalnins, A, Spratt, JC, Garbo, R, Hildick-Smith, D, Elhadad, S, Gagnor, A, Lauer, B, Bryniarski, L, Christiansen, EH, Thuesen, L, Meyer-Geßner, M, Goktekin, O, Carlino, M, Louvard, Y, Lefèvre, T, Lismanis, A, Gelev, VL, Serra, A, Marzà, F, Di Mario, C, Reifart, N & Euro CTO Club 2015, 'Retrograde Recanalization of Chronic Total Occlusions in Europe: Procedural, In-Hospital, and Long-Term Outcomes From the Multicenter ERCTO Registry', Journal of the American College of Cardiology, vol. 65, no. 22, pp. 2388-400. https://doi.org/10.1016/j.jacc.2015.03.566

Arlt, H, Reggiori, F & Ungermann, C 2015, 'Retromer and the dynamin Vps1 cooperate in the retrieval of transmembrane proteins from vacuoles', Journal of Cell Science, vol. 128, no. 4, pp. 645-55. https://doi.org/10.1242/jcs.132720

Klinger, SC , Siupka, P & Nielsen, MS 2015, 'Retromer-Mediated Trafficking of Transmembrane Receptors and Transporters', Membranes, vol. 5, no. 3, pp. 288-306. https://doi.org/10.3390/membranes5030288

Madsen, LHG 2015, Retsdogmatisk forskning i praksis. 4 edn. <http://www.lhgm.dk>

Michelsen, A 2015, Retskilder. in Lærebog om indkomstskat. 16. edn, Djøf Forlag, Kbh., pp. 107-155.

Søndergaard, S 2015, 'Retslægerådet: en analyse af domstolenes anvendelse af Retslægerådet i sager om personskadeerstatning', Aarhus.

Dalberg-Larsen, J 2015, Retssproget, juridisk magtudøvelse og demokrati. in AL Kjær, L Adrian, CB Cederstrøm, J Engberg, J Gabrielsen, M Rosenmeier & S Shaumburg-Müller (eds), Retten i sproget: Samspillet mellem ret og sprog i juridisk praksis. Djøf Forlag, København, pp. 61-72.

Dalberg-Larsen, J 2015, Retsteori og samfundsudvikling. in T Baumbach, P Blume & MG (eds), Ret på flere felter: Forvaltning, Governance, Retssikkerhed. Djøf Forlag, København, pp. 217-232.

Michelsen, A 2015, Rette indkomstmodtager og lovhjemlet indkomstdeling. in Lærebog om indkomstskat. 16. edn, Djøf Forlag, Kbh., pp. 675-711.

Michelsen, A 2015, 'Rette indkomstmodtager - Retmæssig ejer (Beneficial owner) - Misbrug - Bevis - Ansvar for manglende indbetaling af skat', Revision & Regnskabsvæsen, no. 1, RR.SM.2015.800, pp. 75-79.

Boutrup, S 2015, 'Rettelsesblad til NOVANA 2011-2015, Programbeskrivelse del 2', 4 p..

Kjær, AL, Adrian, L, Cederstrøm, CB, Engberg, J , Gabrielsen, J, Rosenmeier, M & Schaumburg-Müller, S (eds) 2015, Retten i sproget. Samspillet mellem ret og sprog i juridisk praksis. Jurist- og Økonomforbundets Forlag, København.

Displaying results 166651 to 166700 out of 389685

3334