Aarhus University Seal

Luminosa

The Luminosa microscope by PicoQuant is a state of the art picosecond time-resolved multi-color single molecule confocal instrument that allows for multi-parameter microscopy to simultaneously report on multi-color fluorescence intensity, lifetime and polarization, and FRET. The microscope allows for a view of sub-millisecond structural dynamics and integrity of biomolocules and macromolecular complexes. It also allows fluorescence intensity and fluorescence lifetime imaging (FLIM) in three dimensions, for example of cells. The Luminosa imaging software allows for initial data analysis during acquisition of data for FLIM and single-molecule specimens. 

If you are interested or have additional questions, please contact us. 

Methodologies

  • smFRET
  • TCSPC
  • FCS
    • FCCS
    • FLCS
  • FLIM
  • FLIM-FRET
  • Anisotropy

Examples of Applications

  • Animal cells
  • Plant cells
  • Biofilms
  • Dynamic structural biology
    • Nucleic acids
    • Proteins
  • Phase separation

Lasers

Diode lasers Pulse width Max. power [40 MHz]
405 nm 48 ps 0.4 mW
485 nm 76 ps 1.1 mW
530 nm 64 ps 4.4 mW
640 nm 92 ps 1.3 mW

Acquisition Modes

  • Fluorescence

  • DIC

  • Transmission

Other Specifications

  • Objective: 60x Water (NA = 1.2)
  • Detectors: 4
  • Scanning: FLIMbee galvo scanner
  • Focus: Autofocus
  • Imaging Software: Luminosa

Filters

  • BP445/30

  • BP511/20

  • BP520/35

  • BP582/64

  • BP690/70

  • BP700/75

  • LP635

  • LP488


Luminosa is located at iNANO, Aarhus University