The MicroMirror TIRF (mmTIRF) microscope is a cutting-edge multi-color, single-molecule total internal reflection fluorescence (TIRF) system designed for high-resolution surface-sensitive fluorescence imaging. Housed within the ASiMoF infrastructure at iNANO, this microscope enables precise and dynamic investigations of molecular interactions at or near surfaces.
The mmTIRF system allows both for multi-color FRET and colocalization single-molecule spectroscopy (CoSMoS technique), offering a powerful platform for studying the organization and behavior of complex biomolecular assemblies. The mmTIRF is equiped with a nanopositioning stage delivering sub-nanometer precision and a TIRF-lock drift stabilization system in the z direction, which is ensuring long-term stability and reproducibility, essential for single-molecule experiments.
The mmTIRF microscope supports one to four color Alternating Laser Excitation (ALEX), enabling the detection and analysis of multiple molecular species simultaneously. This makes it ideally suited for studying dynamic biomolecular interactions and conformational changes with high spatial and temporal resolution. Molecular interactions at the cell-surface interface is additionally an option for investigation.
Whether your interest lies in observing surface-tethered molecular complexes, tracking individual molecules, or performing quantitative co-localization analysis, the mmTIRF offers the tools to do so with exceptional accuracy.
If you are interested or have additional questions, please contact us.
