Jeppesen, D. K., Nawrocki, A.
, Jensen, S. G., Thorsen, K., Whitehead, B., Howard, K., Andersen, L. D., Orntoft, T. F., Larsen, M. R.
& Ostenfeld, M. S. (2014).
Quantitative proteomics of fractionated membrane and lumen exosome proteins from isogenic metastatic and nonmetastatic bladder cancer cells reveal differential expression of EMT factors.
Proteomics,
14(6), 699-712.
https://doi.org/10.1002/pmic.201300452
Miclaus, T., Bochenkov, V., Ogaki, R., Howard, K. & Sutherland, D. S. (2014).
Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes.
Nano Letters,
14(4), 2086-2093.
https://doi.org/10.1021/nl500277c
Whitehead, B. J., Hvam, M. L., Bienk, K., Ostenfeld, M. S. & Howard, K. (2014).
The Application of a Fluorescent Pre-Labelling Method for Evaluation of Exosome Biodistribution. Poster session presented at The 41st Annual Meeting & Exposition of the Controlled Release Society, Chicago, United States.
Li, Y. Y., Femenias, M. R., Aslan, H. H., Yu, Y. Y.
, Howard, K., Dong, MD., Besenbacher, F. F. & Chen, M. M. (2014).
Ultraporous interweaving electrospun microfibers from PCL-PEO binary blends and their inflammatory responses.
Nanoscale,
6(6), 3392-3402.
https://doi.org/10.1039/c3nr06197c
Martirosyan, A., Olesen, M. J., Fenton, R. A., Kjems, J. & Howard, K. A. (2016).
Mucin-mediated nanocarrier disassembly for triggered uptake of oligonucleotides as a delivery strategy for the potential treatment of mucosal tumours.
Nanoscale,
8(25), 12599-12607.
https://doi.org/10.1039/c5nr07206a
Ostenfeld, M. S., Jeppesen, D. K., Bramsen, J. B., Laurberg, J. R., Howard, K., Dyrskjøt, L. & Ørntoft, T. F. (2015).
Selective cellular removal of miR23b by Rab27a/b-dependent exosome release is linked to acquisition of a metastatic property in bladder cancer. Cancer Research.
Whitehead, B. J., Wu, L.
, Hvam, M. L., Aslan, H., Dong, M., Dyrskjøt, L., Ostenfeld, M. S., Moein Moghimi, S.
& Howard, K. (2015).
Tumour exosomes display different differential mechanical and complement activation properties dependent on malignant state: implications in endothelial leakiness.
Journal of Extracellular Vesicles,
4, Article 29685.
https://doi.org/10.3402/jev.v4.29685
Ebbesen, M. F., Olesen, M. T. J., Gjelstrup, M. C., Pakula, M. M., Larsen, E. K., Hansen, I. M., Hansen, P. L., Mollenhauer, J., Malle, B. M.
& Howard, K. (2015).
Tunable CD44-Specific Cellular Retargeting with Hyaluronic Acid Nanoshells.
Pharmaceutical Research,
32(4), 1462-1474.
https://doi.org/10.1007/s11095-014-1552-7
Bienk, K., Hvam, M. L., Pakula, M. M., Dagnæs-Hansen, F., Wengel, J., Malle, B. M.
, Kragh-Hansen, U., Cameron, J., Bukrinski, J. T.
& Howard, K. (2016).
An albumin-mediated cholesterol design-based strategy for tuning siRNA pharmacokinetics and gene silencing.
Journal of Controlled Release,
232, 143-151.
https://doi.org/10.1016/j.jconrel.2016.04.013
Gaglianone, N., Hvam, M. L., Aslan, H., Dong, M., Howard, K. & Ho, Y.-P. (2016).
Chip-Free Microscale-Incubator-Based Synthesis of Chitosan-Based Gene Silencing Nanoparticles.
Particle & Particle Systems Characterization,
33(5), 279-285.
https://doi.org/10.1002/ppsc.201500225
Viuff, D., Antunes, F., Evans, L., Cameron, J.
, Dyrnesli, H., Ravn, B. T.
, Stougaard, M., Thiam, K., Andersen, B., Kjærulff, S.
& Howard, K. (2016).
Generation of a Double Transgenic Humanised Neonatal Fc Receptor (FcRn)/Albumin Mouse to Study the Pharmacokinetics of Albumin-linked Drugs.
Journal of Controlled Release,
223, 22-30.
https://doi.org/10.1016/j.jconrel.2015.12.019
Li, Z., Hu, Y., Chang, M.
, Howard, K., Fan, X.
, Sun, Y., Besenbacher, F. & Yu, M. (2016).
Highly porous PEGylated Bi2S3 nano-urchins as a versatile platform for in vivo triple-modal imaging, photothermal therapy and drug delivery.
Nanoscale,
8(35), 16005-16016.
https://doi.org/10.1039/C6NR03398A
Li, Z., Hu, Y., Jiang, T.
, Howard, K., Li, Y., Fan, X., Sun, Y.
, Besenbacher, F. & Yu, M. (2016).
Human serum albumin coated Prussian blue nanoparticles as pH-/thermo-triggered drug delivery vehicles for cancer thermo-chemotherapy.
Particle & Particle Systems Characterization,
33(1), 53-62.
https://doi.org/10.1002/ppsc.201500189
Li, Z., Hu, Y.
, Howard, K., Yiang, T., Fan, X., Miao, Z., Sun, Y.
, Besenbacher, F. & Yu, M. (2016).
Multifunctional bismuth selenide nanocomposites for anti-tumor thermo-chemotherapy and imaging.
A C S Nano,
10(1), 984-997.
https://doi.org/10.1021/acsnano.5b06259
Li, Z., Liu, J.
, Howard, K., Fan, X., Chang, M., Sun, Y.
, Besenbacher, F. & Yu, M. (2016).
Multimodal Imaging-Guided Antitumor Photothermal Therapy and Drug Delivery Using Bismuth Selenide Spherical-Sponge.
A C S Nano,
10(10), 9646–9658.
https://doi.org/10.1021/acsnano.6b05427
Petersen, S., Kläning, E., Andersen, B., Cameron, J.
, Sørensen, E. S. & Howard, K. (2016).
Neonatal Fc Receptor Binding Tolerance toward the Covalent Conjugation of Payloads to Cysteine 34 of Human Albumin Variants.
Molecular Pharmaceutics,
13(2), 677–682.
https://doi.org/10.1021/acs.molpharmaceut.5b00605
Cai, Y., López-Ruiz , E., Wengel, J., Creemers, L.
& Howard, K. (2017).
A hyaluronic acid-based hydrogel enabling CD44-mediated chondrocyte binding and gapmer oligonucleotide release for modulation of gene expression in osteoarthritis.
Journal of Controlled Release,
253(May), 153-159.
https://doi.org/10.1016/j.jconrel.2017.03.004
Caspersen , M. B.
, Kuhlmann, M., Nicholls , K., Saxton , M. J., Andersen , B., Bunting , K., Cameron, J.
& Howard, K. (2017).
Albumin-based drug delivery using cysteine 34 chemical conjugates; important considerations and requirements.
Therapeutic Delivery,
8(7), 511-519.
https://doi.org/10.4155/tde-2017-0038
Kuhlmann, M., Hamming, J. B. R., Voldum, A., Tsakiridou, G., Larsen, M. T., Schmøkel, J., Sohn, E., Bienk, K., Schaffert, D. H., Sørensen, E. S., Wengel, J.
, Dupont, D. M. & Howard, K. (2017).
An albumin-oligonucleotide assembly for potential combinatorial drug delivery and half-life extension applications.
Molecular Therapy - Nucleic Acids,
9, 284-293.
https://doi.org/10.1016/j.omtn.2017.10.004
Schmidt, E. G. W.
, Hvam, M. L., Antunes, F., Cameron, J., Viuff, D., Andersen, B., Kristensen, N. N.
& Howard, K. A. (2017).
Direct demonstration of a neonatal Fc receptor (FcRn)-driven endosomal sorting pathway for cellular recycling of albumin.
Journal of Biological Chemistry,
292(32), 13312-13322.
https://doi.org/10.1074/jbc.M117.794248
Hvam, M. L., Cai, Y., Dagnæs-Hansen, F., Nielsen, J., Wengel, J.
, Kjems, J. & Howard, K. (2017).
Fatty acid-modified gapmer antisense oligonucleotide and human serum albumin constructs for pharmacokinetic modulation.
Molecular Therapy,
25(7), 1710-1717.
https://doi.org/10.1016/j.ymthe.2017.05.009
Hansen, I., Ebbesen, M. F., Kaspersen, L., Thomsen, T. B., Bienk, K., Cai, Y., Malle, B. M.
& Howard, K. (2017).
Hyaluronic Acid Molecular Weight-Dependent Modulation of Mucin Nanostructure for Potential Mucosal Therapeutic Applications.
Molecular Pharmaceutics,
14(7), 2359–2367.
https://doi.org/10.1021/acs.molpharmaceut.7b00236
Blans, K. I. M., Hansen, M. S., Sørensen, L. V.
, Hvam, M. L., Howard, K., Möller, A., Wiking, L., Larsen, L. B. & Rasmussen, J. T. (2017).
Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography.
Journal of Extracellular Vesicles,
6(1), Article 1294340.
https://doi.org/10.1080/20013078.2017.1294340
Schmøkel, J., Voldum, A., Tsakiridou, G., Kuhlmann, M., Cameron, J.
, Sørensen, E. S., Wengel, J.
& Howard, K. (2017).
Site-selective conjugation of an anticoagulant aptamer to recombinant albumins and maintenance of neonatal Fc receptor binding.
Nanotechnology,
28(20), Article 204004 .
https://doi.org/10.1088/1361-6528/aa6a9b
Christiansen, S. H., Murphy, R. A.
, Juul-Madsen, K., Fredborg, M., Hvam, M. L., Axelgaard, E., Skovdal, S. M., Meyer, R. L., Sorensen, U. B. S., Moeller, A., Nyengaard, J. R., Norskov-Lauritsen, N., Wang, M., Gadjeva, M.
, Howard, K. A., Davies, J. C.
, Petersen, E. & Vorup-Jensen, T. (2017).
The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria.
Scientific Reports,
7(1), Article 15653.
https://doi.org/10.1038/s41598-017-15969-3
Christiansen, S. H., Zhang, X., Juul-Madsen, K., Hvam, M. L., Vad, B. S., Behrens, M. A., Thygesen, I. L., Jalilian, B., Pedersen, J. S., Howard, K., Otzen, D. E. & Vorup-Jensen, T. (2017).
The random co-polymer glatiramer acetate rapidly kills primary human leukocytes through sialic-acid-dependent cell membrane damage.
B B A - Biomembranes,
1859(3), 425-437.
https://doi.org/10.1016/j.bbamem.2017.01.001
Larsen, M. T., Rawsthorne, H.
, Schelde, K. K., Dagnæs-Hansen, F., Cameron, J.
& Howard, K. (2018).
Cellular recycling-driven in vivo half-life extension using recombinant albumin fusions tuned for neonatal Fc receptor (FcRn) engagement.
Journal of Controlled Release,
287, 132-141.
https://doi.org/10.1016/j.jconrel.2018.07.023
Schelde, K. K., Nicholls , K.
, Dagnæs-Hansen, F., Bunting , K., Rawsthorne, H., Andersen, B., Finnis, C., Williamson, M., Cameron, J.
& Howard, K. (2019).
A new class of recombinant human albumin with multiple surface thiols exhibit stable conjugation, and enhanced FcRn binding and blood circulation.
Journal of Biological Chemistry,
294(10), 3735-3743.
https://doi.org/10.1074/jbc.RA118.005870
Garcia, JP., Stein, J.
, Cai, Y., Riemers, F., Wexselblatt, E., Wengel, J., Tryfonidou, M., Yayon, A.
, Howard, K. & Creemers, L. B. (2019).
Fibrin-hyaluronic acid hydrogel-based delivery of antisense oligonucleotides for ADAMTS5 inhibition in co-delivered and resident joint cells in osteoarthritis. Journal of Controlled Release,
294, 247-258.
https://doi.org/10.1016/j.jconrel.2018.12.030
Fredsøe, J., Rasmussen, A. K. I.
, Laursen, E. B., Cai, Y., Howard, K. A., Pedersen, B. G., Borre, M., Mouritzen, P.
, Òrntoft, T. & Sørensen, K. D. (2019).
Independent Validation of a Diagnostic Noninvasive 3-MicroRNA Ratio Model (uCaP) for Prostate Cancer in Cell-Free Urine.
Clinical Chemistry,
65(4), 540-548.
https://doi.org/10.1373/clinchem.2018.296681
Nandakumar, R., Tschismarov, R., Meissner, F.
, Prabakaran, T., Krissanaprasit, A., Farahani, E., Zhang, B. C., Assil, S., Martin, A., Bertrams, W.
, Holm, C. K., Ablasser, A.
, Klause, T., Thomsen, M. K., Schmeck, B.
, Howard, K. A., Henry, T.
, Gothelf, K. V., Decker, T.
& Paludan, S. R. (2019).
Intracellular bacteria engage a STING–TBK1–MVB12b pathway to enable paracrine cGAS–STING signalling.
Nature Microbiology,
4, 701-713.
https://doi.org/10.1038/s41564-019-0367-z
Hall, A., Nicholls , K., Caspersen , M. B.
, Skrivergaard, S., Howard, K., Karu, K., Chudasama, V. & Baker, J. R. (2019).
Optimised approach to albumin-drug conjugates using monobromomaleimide-C-2 linkers.
Organic & Biomolecular Chemistry,
17(34), 7870-7873.
https://doi.org/10.1039/C9OB00721K
Larsen, M. T., Mandrup, O. A., Schelde, K. K., Luo, Y., Sørensen, K. D., Dagnæs-Hansen, F., Cameron, J.
, Stougaard, M., Steiniche, T. & Howard, K. A. (2020).
FcRn overexpression in human cancer drives albumin recycling and cell growth: a mechanistic basis for exploitation in targeted albumin-drug designs.
Journal of Controlled Release,
322, 53-63.
https://doi.org/10.1016/j.jconrel.2020.03.004
Kania, K., Colella, F., Riemen, A. H. K., Wang, H.
, Howard, K., Aigner, T., Dell’Accio, F., Capellini, T. D., Roelofs, A. J. & De Bari, C. (2020).
Regulation of Gdf5 expression in joint remodelling, repair and osteoarthritis.
Scientific Reports,
10(1), 157. Article 157.
https://doi.org/10.1038/s41598-019-57011-8
Juul-Madsen, K., Qvist, P., Bendtsen, K. L., Langkilde, A. E., Vestergaard, B.
, Howard, K., Dehesa-Etxebeste, M.
, Paludan, S. R., Andersen, G. R., Jensen, P. H., Otzen, D. E., Romero-Ramos, M. & Vorup-Jensen, T. (2020).
Size-Selective Phagocytic Clearance of Fibrillar α-Synuclein through Conformational Activation of Complement Receptor 4.
Journal of Immunology,
204(5), 1345-1361.
https://doi.org/10.4049/jimmunol.1900494