Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Paravar, A, Hay, FR, Abdollahi, F & Pritchard, HW 2026, 'Assessment of Physio-biochemical Processes During Seed Filling in Lallemantia Species Under Drought Stress', Journal of Plant Growth Regulation, vol. 45, no. 1, pp. 780-800. https://doi.org/10.1007/s00344-025-11906-1

Ali, Z, Thomsen, K , Vestergaard, C & Thomsen, SF 2026, 'Assessment of Quality and Utility of Patient-Taken Smartphone Photographs of Atopic Dermatitis: Clinical Survey Study', JMIR dermatology, vol. 9, e72916. https://doi.org/10.2196/72916

Lashkari, S 2026, 'Assessment of safety of milk replacer', No. 2026-0940640, 1 p., Feb 03, 2026. Rådgivningsnotat fra DCA - Nationalt Center for Fødevarer og Jordbrug.

Romero, D, Appendini, CM, Emanuel, K, Lee, C-Y, Nederhoff, K, Bloemendal, N, Ruiz-Salcines, P, Vigh, J & Dominguez, C 2026, 'Assessment of synthetic tropical cyclones in the North Atlantic basin', Atmospheric Research, vol. 327, 108404. https://doi.org/10.1016/j.atmosres.2025.108404

Nielsen, JJJ , Levison, LS & Andersen, H 2026, 'Associated Autoimmunity in Myasthenia Gravis in Denmark: A Nationwide Case–Control Study', European Journal of Neurology, vol. 33, no. 2, e70498. https://doi.org/10.1111/ene.70498

Agervig, F, Gedde, AR, Østergård, AV, Nettinger, M, Müller, SD, Nielsen, RP, Nadkarni, VM, Wittig, J , Løfgren, B & Glerup Lauridsen, K 2026, 'Association between barriers of resuscitation and chest compression quality metrics during in-hospital cardiac arrest resuscitation attempts', Resuscitation, vol. 221, 111030. https://doi.org/10.1016/j.resuscitation.2026.111030

Ørnberg, E, Bruun, V, Subhi, Y, Anguita, R, Cehofski, LJ, Desideri, LF, van Dijk, EHC, Hansen, MS, Klefter, ON, Muttuvelu, DV, Petersen, L, Sabaner, MC, Schneider, M, Sevik, MO & Subhi, Y 2026, 'Association between central serous chorioretinopathy and personality types: A systematic review and meta-analysis', Acta Ophthalmologica. https://doi.org/10.1111/aos.70097

Ge, F , Wang, Y , Liu, X , Munk-Olsen, T , Madsen, KB , Pedersen, EM , Albiñana, C , Agerbo, E, Bulik, CM, Petersen, LV, Valdimarsdottir, UA & Vilhjálmsson, BJ 2026, 'Association Between Maternal Genome-Wide Polygenic Scores for Psychiatric and Neurodevelopmental Disorders and Adverse Perinatal Events: A Danish Population-Based Study', Biological Psychiatry Global Open Science, vol. 6, no. 1, 100613. https://doi.org/10.1016/j.bpsgos.2025.100613

Ladefoged, BT , Olesen, KK , Maeng, M & Poulsen, SH 2026, 'Association Between Occupational Physical Work Exposure and Wild-Type Transthyretin Cardiomyopathy', JACC: Advances, vol. 5, no. 3, pp. 102619. https://doi.org/10.1016/j.jacadv.2026.102619

Hjort, PB , Hyldgaard, JM, Young-Halvorsen, K, Hüsch, T, Jensen, JB & Ernst, A 2026, 'Association Between Photodynamic Diagnosis and Risk of Recurrence and Progression in Bacillus Calmette-Guérin-treated Nonmuscle Invasive Bladder Cancer Patients: A Nationwide Follow-up Study', Urology. https://doi.org/10.1016/j.urology.2026.02.004

Böttcher, MH, Bossen, L , Mellemkjær, A, Holland-Fischer, PH, Bach Hansen, J, Møller, HJ & Grønbæk, H 2026, 'Association between quality of life and inflammation and fibrosis markers in primary biliary cholangitis over three-year follow-up', Scandinavian Journal of Gastroenterology, vol. 61, no. 1, pp. 133-142. https://doi.org/10.1080/00365521.2025.2602520

Alkner, S, Appelgren, M, Szulkin, R, Wieslander, E, Rogowski, V, Gustafsson, CJ, Nissen, HD, Sackey, H, Lundstedt, D, Andersson, Y, Bergkvist, L, Frisell, J, Olofsson Bagge, R, Sund, M, Rydén, L, Offersen, BV & de Boniface, J 2026, 'Association between shoulder joint radiation dose and arm morbidity in the randomized breast cancer trial SENOMAC', Radiotherapy and Oncology, vol. 218, 111454. https://doi.org/10.1016/j.radonc.2026.111454

Borges, GH, Vinhal Henriques, I, de Andrade Vieira, W, Spin-Neto, R & Paranhos, LR 2026, 'Association between tooth agenesis and root morphology assessed by periapical radiographs: Retrospective cross-sectional observational study', Journal of Orofacial Orthopedics. https://doi.org/10.1007/s00056-025-00639-1

Kader, K, Rasmussen, LD , Karim, SR , Westra, J , Isaksen, C , Søby, JH , Dahl, JN , Brix, L , Petersen, SE, Murphy, T, Winther, S , Christiansen, EH , Böttcher, M & Eftekhari, A 2026, 'Association of left ventricular mass with discordant stress cardiac magnetic resonance and coronary angiography', European Heart Journal Cardiovascular Imaging. https://doi.org/10.1093/ehjci/jeaf350

Ganesh, A, Gaist, D, Modrau, B , Gude, MF , Behrndtz, AB , Andersen, G , Simonsen, CZ & Blauenfeldt, R 2026, 'Association of prehospital treatment duration with the efficacy of remote ischemic conditioning in the RESIST trial', International Stroke Conference 2026, 04/02/2026.

Hjelholt, TJ , Veedfald, T, Frølich, A, Appel, C , Thomsen, HH , Prior, A , Bjerrum, AD, Balsby, M, Jensen, AMS & Nielsen, LK 2026, 'Association of the Tilburg Frailty Indicator with mortality, readmission, and healthcare contacts in adult patients with multimorbidity', Journal of multimorbidity and comorbidity, vol. 16. https://doi.org/10.1177/26335565251415477

Just-Nørregaard, V , Dalgaard, VLP , Bruun, JM , Andersen, JH & Winding, TN 2026, 'Associations between perceived stress, psychosocial stressors, and HbA1c levels in healthy young adults from a prospective cohort study', Scientific Reports, vol. 16, no. 1, 4897. https://doi.org/10.1038/s41598-026-35066-8

Hansen, A-KL , Jacobsen, CB & Dalgaard, VLP 2026, 'Associations between psychosocial work environment factors and the well-being of frontline hospital managers: A cross-sectional study', Health Care Management Review.

Søndergaard, A, Hemager, N, Wilms, M, Rohd, SB, Krantz, MF, Greve, AN , Veddum, L, Thorup, AAE, Nordentoft, M & Gregersen, M 2026, 'Associations between Sleep Problems, Psychotic Experiences and Mental Disorders in Children at Familial High Risk of Schizophrenia or Bipolar Disorder and Population-based controls: The Danish High Risk and Resilience Study', Schizophrenia Research, vol. 291, pp. 99-106. https://doi.org/10.1016/j.schres.2026.02.020

Pereira, LDL, Herkrath, FJ, Siqueira, JH, Leal, MDC, Abreu, FMD, Lopes, AF & Vettore, MV 2026, 'Associations between socioeconomic status, dietary habits and health-related quality of life among children in rural riverside communities: the mediation role of food insecurity', Quality of Life Research, vol. 35, no. 2, 41. https://doi.org/10.1007/s11136-025-04137-0

Sørensen, KH, Levinsen, AKG, Kjaer, TK, Borre, M, Brasso, K, Fode, M, Dalton, SO & Larsen, SB 2026, 'Associations Between Stress Urinary Incontinence and Self-reported Functioning and Urinary Symptoms Among Survivors of Prostate Cancer Treated With Radical Prostatectomy', Urology, vol. 207, pp. 207-211. https://doi.org/10.1016/j.urology.2025.10.021

Özcan, D , Nielsen, PS , Alsner, J , Nielsen, MH, Maae, E, Milo, MLH , Overgaard, J , Offersen, BV & Tramm, T 2026, 'Associations between tumor immune response and prognosis in node-negative breast cancer patients in the randomized DBCG HYPO trial', BMC Cancer. https://doi.org/10.1186/s12885-026-15859-w

Herraiz-Adillo, Á, Ahlqvist, VH, Hedman, K, Higueras-Fresnillo, S, Hagström, E, de Smidt, MF, Daka, B, Lenander, C, Olsson, A, Berglind, D, Östgren, CJ, Rådholm, K, Ortega, FB & Henriksson, P 2026, 'Associations of adolescent BMI and physical fitness with cardiovascular health in middle age: a population-based prospective study of Swedish men', American Journal of Preventive Medicine, vol. 70, no. 4, 108128, pp. 108128. https://doi.org/10.1016/j.amepre.2025.108128

Hakulinen, C, Gutvilig, M, Niemi, R, Momen, NC, Pulkki-Råback, L, Böckerman, P, Plana-Ripoll, O, Komulainen, K & Elovainio, M 2026, 'Associations of mental disorders in children with parents' subsequent mental disorders: nationwide cohort study from Finland and Denmark', The British journal of psychiatry : the journal of mental science, vol. 228, no. 1, pp. 10-17. https://doi.org/10.1192/bjp.2024.249

Bardel, B, Lefaucheur, JP, Mussigmann, T, Bapst, B, Senova, S, Tankisi, H & Fanella, G 2026, 'A Standardized Protocol for Functional Motor Mapping Using Navigated Transcranial Magnetic Stimulation', Journal of Visualized Experiments, vol. 2026-February, no. 228, e69776. https://doi.org/10.3791/69776

Scutt, OJ, Davies, GR, Stokholm, A, Lyttle, AJ, Nielsen, MB, Hatt, E, Li, T, Lund, MN & Bedding, TR 2026, 'Asteroseismology of solar-like oscillators: emulating individual mode frequencies with a branching neural network', Monthly Notices of the Royal Astronomical Society, vol. 546, no. 4, stag018. https://doi.org/10.1093/mnras/stag018

Faldborg, KB 2026, 'A Study in Healthy Volunteers to Improve Methods for Detection of GHB in Victims of Drug-Facilitated Sexual Assault', PhD, Aarhus University.

Salazar, LC, Baxter, EM, Lawrence, AB, Moses, T, Thomson, JR & D'Eath, RB 2026, 'A study of associations between the salivary metabolite composition, pH, and gastric ulcers in lactating sows', Animal : an international journal of animal bioscience, vol. 20, no. 3, 101774. https://doi.org/10.1016/j.animal.2026.101774

Laugesen, AK, Køhler, E, Buhl, CS & Brincks, JK 2026, 'A study of the reliability and validity of the Six-Spot Step Test Cognitive in ambulatory people with diabetic peripheral neuropathy', Clinical Rehabilitation, vol. 40, no. 4, pp. 486-497. https://doi.org/10.1177/02692155251397583

Stærke, NB, Rudolfsen, JH, Olsen, J, Skovdal, M & von Linstow, ML 2026, 'A survey-based analysis of extended parental leave and work or educational absenteeism in Denmark due to respiratory syncytial virus in hospitalised infants', BMC Public Health, vol. 26, no. 1, 201. https://doi.org/10.1186/s12889-025-25926-9

Li, M, Zhang, P, Xing, W, Zheng, Y, Zaporojets, K, Chen, J, Zhang, R, Zhang, Y, Gong, S, Hu, J, Ma, X, Liu, Z, Groth, P & Worring, M 2026, 'A survey of large language models for data challenges in graphs', Expert Systems with Applications, vol. 298, Part A, 129643. https://doi.org/10.1016/j.eswa.2025.129643

Khawaja, W, Ezuma, M, Semkin, V, Erden, F, Ozdemir, O & Guvenc, I 2026, 'A Survey on Detection, Classification, and Tracking of AAVs Using Radar and Communications Systems', IEEE Communications Surveys and Tutorials, vol. 28, pp. 3272-3310. https://doi.org/10.1109/COMST.2025.3554613

Ni, M, Luo, P, Yang, H, Cheng, Y, Li, H & Huang, Y 2026, 'Asynchronous shifts in plant ecological strategies across forest layers during succession on Hengduan mountains', Ecological Indicators, vol. 182, 114579. https://doi.org/10.1016/j.ecolind.2025.114579

Joseph, JV , Blaavand, MS, Kelstrup, GW, Rasmussen, SA , Cai, H , Vendelbo, M & Thomsen, MK 2026, 'A syngeneic glioblastoma mouse model with mesenchymal hallmarks', Neuroscience, vol. 600, pp. 26-33. https://doi.org/10.1016/j.neuroscience.2026.02.018

Fuhlert, P, Westhaeusser, F, Dietrich, E, Lennartz, M, Khatri, R, Kaiser, N, Röbeck, P, Bülow, R, Von Stillfried, S, Witte, A, Ladjevardi, S, Drotte, A, Severgårdh, P, Baumbach, J, Puelles, VG, Häggman, M, Brehler, M, Boor, P, Walhagen, P, Dragomir, A, Busch, C, Graefen, M, Bengtsson, E, Sauter, G, Zimmermann, M & Bonn, S 2026, 'A systematic analysis of the impact of data variation on AI-based histopathological grading of prostate cancer', Medical Image Analysis, vol. 108, 103884. https://doi.org/10.1016/j.media.2025.103884

Pade, M 2026, A Tacitus from the Library of Agostino Maffei. a Fifteenth-Century Italian Manuscript now in the Royal Library of Copenhagen, GkS 496 2o. in P Carmassi & O Merisalo (eds), Libraries lost - Libraries recovered. : Dispersion, Reconstitution and Reconstruction of Libraries since the Renaissance,. Brepols, Bibliologia.

Homrighausen, S 2026, 'A Tale of Social Choice Mechanisms', PhD, Aarhus University.

Eriksson, B 2026, 'At blive kvinde og hade det', Litteraturmagasinet Standart, vol. 40, no. 1, pp. 36-38.

Skovgaard, AKP 2026, At følge formen i stoffet: Om vævens virkning i Anna Thommesens arbejde. in Anna Thommesen: Vævninger / Weavings. Strandberg Publishing, København, pp. 142-159.

Petersen, AK 2026, A Theory of Innovation in Cultural and Social Studies Exemplified by Changes in Judean Religion. in SL Jónsson, S Luther & JPB Mortensen (eds), Innovation and Appropriation in Early Christianity: Authors, Topics, Texts, Genres. Vandenhoeck & Ruprecht, Göttingen, Studia Aarhusiana Neotestamentica (SANt), vol. 11, pp. 73-114.

Whiteman, IT, Cook, AL, Augustine, EF, Bindoff, AD, Johnson, AM, Mason, HL, Mink, JW, Østergaard, JR, Schulz, A, Vermilion, J, Vierhile, A & Adams, HR 2026, 'A timeline of symptom onset and disease progression in CLN3 disease', Orphanet Journal of Rare Diseases, vol. 21, no. 1, 38. https://doi.org/10.1186/s13023-025-04174-5

McCutcheon, J, McQuaid, JB, Canha, N, Barr, SL, Lutz, S, Roddatis, V, Mayanna, S, Tedstone, AJ, Tranter, M & Benning, LG 2026, 'Atmospheric Deposition of Local Mineral Dust Delivers Phosphorus to the Greenland Ice Sheet', Environmental Science & Technology, vol. 60, no. 3, pp. 2515-2527. https://doi.org/10.1021/acs.est.5c13873

Iskandar, RJ, Mailhac, A, Mulick, A, Matthewman, J, Abuabara, K, Deleuran, M , Vestergaard, C , Sørensen, HT, Langan, SM & Schmidt, SAJ 2026, 'Atopic Dermatitis and Academic Performance', JAMA Dermatology. https://doi.org/10.1001/jamadermatol.2026.0425

Kjersgaard, CL , Gaml-Sørensen, A , Clemmensen, PJ , Arendt, LH, Håberg, SE, Arah, OA , Deleuran, M & Ramlau-Hansen, CH 2026, 'Atopic dermatitis and fecundity: a Danish National Birth Cohort study', Human Reproduction Open, vol. 2026, no. 1, hoaf077. https://doi.org/10.1093/hropen/hoaf077

Ibler, K, Hærskjold, A, Olsen, J, Themstrup, L, Dalager, M, Skov, L, Mortz, CG, Balk-Møller, E & Vestergaard, C 2026, 'Atopic Dermatitis in Denmark: Regional Variation', Acta Dermato-Venereologica, vol. 106, adv44122. https://doi.org/10.2340/actadv.v106.44122

Brøgger, K , Harsbo, R , Moscovitz, H & Zangrandi, E 2026, 'A Topological Comparative Approach: Studying the influence of new nationalisms across different higher education policy sites', Higher Education.

Kaas, JG & Svendsen, GT 2026, 'A Tough-Rider Typology on Informal Coordination Between EU Member States', European Foreign Affairs Review, vol. 31, no. 1. <https://kluwerlawonline.com/journalarticle/European+Foreign+Affairs+Review/31.1+%5Bpre-publication%5D/EERR2026009 >

Kejl, KJONAS & Svendsen, G 2026, 'A Tough-Rider Typology on Informal Coordination Between EU Member States', European Foreign Affairs Review, vol. 31, no. 1 [pre-publication], pp. 1-18.

Wang, M, Zandona, B, Räisänen, SE, Serviento, AM & Niu, M 2026, 'A tracking-by-classification approach for continuous individual monitoring of Holstein dairy cows in a free-stall barn', Biosystems Engineering, vol. 265, 104420. https://doi.org/10.1016/j.biosystemseng.2026.104420

Charney, AW, Liharska, LE, Vornholt, E, Valentine, A, Lund, A, Hashemi, A, Thompson, RC, Lohrenz, T, Johnson, JS, Bussola, N, Cheng, E, Park, YJ, Qasim, S, Aristel, A, Wilkins, L, Ziafat, K, Silk, H, Linares, LM, Sullivan, B, Feng, C, Batten, SR, Bang, D, Barbosa, LS, Twomey, T, White, JP, Vannucci, M, Hadj-Amar, B, Moya, E, Figee, M, Nadkarni, GN, Breen, MS, Kishida, KT, Scarpa, J, Schadt, EE, Saez, I, Montague, PR, Beckmann, ND & Kopell, BH 2026, 'A transcriptional program associated with neurotransmission in the living human brain', Molecular Psychiatry. https://doi.org/10.1038/s41380-025-03420-3

Displaying results 351 to 400 out of 389737

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen