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About iSMS

iSMS is a user-interfaced software package for the visualization and analysis of wide-field, single-molecule FRET (smFRET) microscopy using immobilized molecules.

In smFRET microscopy, the structural conformations and dynamic equilibria of hundreds of single biomolecules are monitored in real-time. This provides us with insight into the conformational landscapes and transitional dynamics of DNA, RNA and proteins, being important for the functioning of these biomolecules. Equally important, smFRET provides a direct view into the population heterogeneity of biological samples which traditionally has been hidden behind an ensemble average.

iSMS is designed to quickly process, analyse and share smFRET data with peers and collaborators. Ultimately we hope to unify and standardize the analysis of smFRET microscopy across different laboratories.

The open-source version is written in MATLAB (Mathworks) but is compiled into a standalone executable software. The compiled version does not require MATLAB nor a MATLAB license.


iSMS is developed in the Single-Molecule Biophotonics Laboratory of Victoria Birkedal at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark.

Feel free to contact us with questions or comments: