Download iSMS for MATLAB (includes source code)

If you need other versions of iSMS, please contact us

Updates in version 2.01

• Inclusion of background estimator toolkits described in: Preus, S., Hildebrand, L L., Birkedal V., "Optimal Background Estimators in Single-Molecule FRET Microscopy", Biophysical Journal (2016), 111, 6, 1278-1286
• Small bugs were fixed in this version

Download iSMS for MATLAB (includes source code)

Download iSMS for Windows 64-bit (not requiring MATLAB)

Download iSMS for OS X (coming soon)

Updates in version 1.06:

• Fixed an error in "backgroundSettings.m" so that the different background settings can be set in "FRET pairs window" now 

1. Installation instructions
2. Download test data below
3. Download demo session below
4. Follow the quick guides
5. Checklist of important parameters
6. Documentation pages

Demo session

1. Download demo session (8 MB)
2. Quick guide on how to open and explore the session

The demo session only contains processed data, such as average images, peak positions, time traces etc. In order to recalculate traces using new settings you will need to download and reload the raw movie files below into the session. Guide on how to reload raw movie data into an existing session

The demo session contains data acquired using alternating laser excitation (ALEX). See this page for the experimental details of the demo data

Raw movie data

• Download raw movie file 1 (zipped 236 MB)
• Download raw movie file 2 (zipped 261 MB)
• Download raw movie file 3 (zipped 522 MB)

These raw movie files are in Andor's SIF-format and acquired using an alternating laser excitation (ALEX) scheme. Other formats are also supported. Guide on how to load raw data into iSMS.

Preus, S., Noer, S.L., Hildebrandt, L.L., Gudnason, D., Birkedal, V., "iSMS: single-molecule FRET microscopy software", Nature Methods (2015) 12, 7, 593–594

Preus, S., Hildebrand, L. L., Birkedal, V., "Optimal Background Estimators in Single-Molecule FRET Microscopy", Biophysical Journal (2016), 111, 6, 1278-1286

Hildebrandt, L. L., Preus, S., Birkedal, V. "Quantitative single molecule FRET efficiencies using TIRF microscopy", Faraday Discussions (2015), 184. 131-142

Version 1.04 - 2015.07.01

Contact Daniel Gudnason or Victoria Birkedal for this version.