Single-Molecule FRET Microscopy Software

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Load Raw Image Data into iSMS

Supported file formats

iSMS supports all raw image file formats implemented in the Bio-Formats Library by the Open Microscopy Environment (OME). This includes >120 image and movie formats from most major microscopy and camera suppliers:

See full list here.

iSMS additionally reads Andor's EMCCD iXon camera .sif file format as produced by the Andor Solis Image Acquisition Software.

Load raw image data

Raw data is loaded in either one of the following ways:

  • File->Data->Load movie files
  • File->Recent movies
  • The '...' button next to the files listbox.
Raw data is loaded using the '...' button in the main window.

Delete data

Files are removed from the current iSMS session using the 'X' button in the main window. This button will also delete all FRET-pairs in the file.

Circumvent memory issues

Raw image data takes up a lot of RAM. The raw image data can, and should, be removed from RAM once all intensity traces have been calculated.

See here how to circumvent memory issues in iSMS.

Revised 17.04.2023