Larsen, E. K. U., Nielsen, T., Wittenborn, T., Rydtoft, L. M., Lokanathan, A. R., Hansen, L., Østergaard, L., Kingshott, P., Howard, K. A., Besenbacher, F., Nielsen, N. C. & Kjems, J. (2012).
Accumulation of magnetic iron oxide nanoparticles coated with variably sized polyethylene glycol in murine tumors.
Nanoscale,
4(7), 2352-2361 .
https://doi.org/10.1039/C2NR11554A
Cai, Y., López-Ruiz , E., Wengel, J., Creemers, L.
& Howard, K. (2017).
A hyaluronic acid-based hydrogel enabling CD44-mediated chondrocyte binding and gapmer oligonucleotide release for modulation of gene expression in osteoarthritis.
Journal of Controlled Release,
253(May), 153-159.
https://doi.org/10.1016/j.jconrel.2017.03.004
Caspersen , M. B.
, Kuhlmann, M., Nicholls , K., Saxton , M. J., Andersen , B., Bunting , K., Cameron, J.
& Howard, K. (2017).
Albumin-based drug delivery using cysteine 34 chemical conjugates; important considerations and requirements.
Therapeutic Delivery,
8(7), 511-519.
https://doi.org/10.4155/tde-2017-0038
Dinesen, A., Winther, A., Wall, A.
, Märcher, A., Palmfeldt, J., Chudasama, V., Wengel, J.
, Gothelf, K. V., Baker, J. R.
& Howard, K. A. (2022).
Albumin Biomolecular Drug Designs Stabilized through Improved Thiol Conjugation and a Modular Locked Nucleic Acid Functionalized Assembly.
Bioconjugate Chemistry,
33(2), 333-342.
https://doi.org/10.1021/acs.bioconjchem.1c00561
Elkhashab, M., Dilek, Y.
, Foss, M., Creemers, L. B.
& Howard, K. A. (2024).
A Modular Albumin-Oligonucleotide Biomolecular Assembly for Delivery of Antisense Therapeutics: Molecular Pharmaceutics.
Molecular Pharmaceutics,
21(2), 491-500.
https://doi.org/10.1021/acs.molpharmaceut.3c00561
Fuchs, E., Rudnik-Jansen, I., Dinesen, A., Selnihhin, D., Mandrup, O. A., Thiam, K.
, Kjems, J., Pedersen, F. S. & Howard, K. A. (2022).
An albumin-angiotensin converting enzyme 2-based SARS-CoV-2 decoy with FcRn-driven half-life extension.
Acta Biomaterialia,
153, 411-418.
https://doi.org/10.1016/j.actbio.2022.09.048
Dinesen, A., Winther, A., Wall, A.
, Märcher, A., Palmfeldt, J., Chudasama, V., Wengel, J.
, Gothelf, K. V., Baker, J. R.
& Howard, K. (2022).
An Albumin Biomolecular Drug Design Stabilized through Improved Thiol Conjugation and a Modular Locked Nucleic Acid Assembly. Poster session presented at Controlled Release Society 2022 Annual Meeting, Montreal, Quebec, Canada.
Dinesen, A., Andersen, V. L., Elkhashab, M., Pilati, D., Bech, P., Fuchs, E., Samuelsen, T. R., Winther, A., Cai, Y., Märcher, A., Wall, A.
, Omer, M., Nielsen, J. S., Chudasama, V., Baker, J. R.
, Gothelf, K. V., Wengel, J.
, Kjems, J. & Howard, K. A. (2024).
An Albumin-Holliday Junction Biomolecular Modular Design for Programmable Multifunctionality and Prolonged Circulation.
Bioconjugate Chemistry,
35(2), 214-222.
https://doi.org/10.1021/acs.bioconjchem.3c00491
Bienk, K., Hvam, M. L., Pakula, M. M., Dagnæs-Hansen, F., Wengel, J., Malle, B. M.
, Kragh-Hansen, U., Cameron, J., Bukrinski, J. T.
& Howard, K. (2016).
An albumin-mediated cholesterol design-based strategy for tuning siRNA pharmacokinetics and gene silencing.
Journal of Controlled Release,
232, 143-151.
https://doi.org/10.1016/j.jconrel.2016.04.013
Kuhlmann, M., Hamming, J. B. R., Voldum, A., Tsakiridou, G., Larsen, M. T., Schmøkel, J., Sohn, E., Bienk, K., Schaffert, D. H., Sørensen, E. S., Wengel, J.
, Dupont, D. M. & Howard, K. (2017).
An albumin-oligonucleotide assembly for potential combinatorial drug delivery and half-life extension applications.
Molecular Therapy - Nucleic Acids,
9, 284-293.
https://doi.org/10.1016/j.omtn.2017.10.004
Nygaard, J. V., Andersen, M. Ø., Howard, K., Sasov, A.
, Kjems, J. & Besenbacher, F. (2004).
Analysis of Nanoparticles in Scaffolds for Tissue Engineering by use of X-Ray Nano Tomography. Poster session presented at iNANO 4
th annual meeting, Aarhus, Denmark.
Schelde, K. K., Nicholls , K.
, Dagnæs-Hansen, F., Bunting , K., Rawsthorne, H., Andersen, B., Finnis, C., Williamson, M., Cameron, J.
& Howard, K. (2019).
A new class of recombinant human albumin with multiple surface thiols exhibit stable conjugation, and enhanced FcRn binding and blood circulation.
Journal of Biological Chemistry,
294(10), 3735-3743.
https://doi.org/10.1074/jbc.RA118.005870
de Paz, L.
, Resin, A., Howard, K., Sutherland, D. S. & Wejse, P. (2011).
Antimicrobial effect of chitosan nanoparticles on streptococcus mutans biofilms.
Applied and Environmental Microbiology,
77(11), 3892-3895.
Rahbek, U. L., Nielsen, A. F., Dong, M., You, Y., Chauchereau, A., Oupicky, D.
, Besenbacher, F., Kjems, J. & Howard, K. A. (2010).
Bioresponsive hyperbranched polymers for siRNA and miRNA delivery.
Journal of Drug Targeting,
18(10), 812-20.
https://doi.org/10.3109/1061186X.2010.527982
Hartmann, H., Lakner, U.
, Andersen, M. Ø., Kjems, J., Howard, K. A. & Schloßhauer, B. (2009).
Carrier Matrix for siRNA-Mediated Gene Knock Down to Promote Neuronal Regeneration. Tissue Engineering. Part A. Tissue Engineering, (3), 718.
Yang, H. Y., Vonk, L. A., Licht, R., Van Boxtel, A. M. G., Bekkers, J. E. J., Kragten, A. H. M.
, Hein, S., Varghese, O. P.
, Howard, K., Cumhur Öner, F., Dhert, W. J. A. & Creemers, L. B. (2014).
Cell type and transfection reagent-dependent effects on viability, cell content, cell cycle and inflammation of RNAi in human primary mesenchymal cells.
European Journal of Pharmaceutical Sciences,
53(1), 35-44.
https://doi.org/10.1016/j.ejps.2013.12.006
Larsen, M. T., Rawsthorne, H.
, Schelde, K. K., Dagnæs-Hansen, F., Cameron, J.
& Howard, K. (2018).
Cellular recycling-driven in vivo half-life extension using recombinant albumin fusions tuned for neonatal Fc receptor (FcRn) engagement.
Journal of Controlled Release,
287, 132-141.
https://doi.org/10.1016/j.jconrel.2018.07.023
Gaglianone, N., Hvam, M. L., Aslan, H., Dong, M., Howard, K. & Ho, Y.-P. (2016).
Chip-Free Microscale-Incubator-Based Synthesis of Chitosan-Based Gene Silencing Nanoparticles.
Particle & Particle Systems Characterization,
33(5), 279-285.
https://doi.org/10.1002/ppsc.201500225
Howard, K. A., Paludan, S. R., Behlke, M. A.
, Besenbacher, F., Deleuran, B. W. & Kjems, J. (2008).
Chitosan/siRNA nanoparticle-mediated TNF-alpha knockdown in peritoneal macrophages for anti-inflammatory treatment in a murine arthritis model.
Molecular Therapy,
17(1), 162-168.
https://doi.org/10.1038/mt.2008.220
Mittnacht, U., Hartmann, H.
, Hein, S., Oliveira, H.
, Dong, M., Pêgo, A. P.
, Kjems, J., Howard, K. A. & Schlosshauer, B. (2010).
Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth.
Nano Letters,
10(10), 3933-9.
https://doi.org/10.1021/nl1016909
Chen, M., Gao, S., Dong, M., Song, J., Yang, C., Howard, K. A., Kjems, J. & Besenbacher, F. (2012).
Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery.
A C S Nano,
6(6), 4835-4844.
https://doi.org/10.1021/nn300106t
Jeppesen, D. K., Hvam, M. L., Primdahl-Bengtson, B.
, Boysen, A. T., Whitehead, B., Dyrskjøt, L., Orntoft, T. F., Howard, K. & Ostenfeld, M. S. (2014).
Comparative analysis of discrete exosome fractions obtained by differential centrifugation.
Journal of Extracellular Vesicles,
3, 1-16. Article 25011.
https://doi.org/10.3402/jev.v3.25011
Andersen, M. Ø., Howard, K. A., Paludan, S. R., Besenbacher, F. & Kjems, J. (2008).
Delivery of siRNA from lyophilized polymeric surfaces.
Biomaterials,
29(4), 506-512.
https://doi.org/10.1016/j.biomaterials.2007.10.003
Schmidt, E. G. W.
, Hvam, M. L., Antunes, F., Cameron, J., Viuff, D., Andersen, B., Kristensen, N. N.
& Howard, K. A. (2017).
Direct demonstration of a neonatal Fc receptor (FcRn)-driven endosomal sorting pathway for cellular recycling of albumin.
Journal of Biological Chemistry,
292(32), 13312-13322.
https://doi.org/10.1074/jbc.M117.794248
Xu, S., Dong, M., Liu, X., Howard, K. A., Kjems, J. & Besenbacher, F. (2007).
Direct force measurements between siRNA and chitosan molecules using force spectroscopy.
Biophysical Journal,
93(3), 952-9.
https://doi.org/10.1529/biophysj.106.093229
Mistry, A.
, Glud, S. Z., Kjems, J., Nyengaard, J. R., Howard, K. A., Stolnik, S. & Illum, L. (2009).
Effect of physicochemical properties on intranasal nanoparticle transit into murine olfactory epithelium.
Journal of Drug Evaluation,
17(7), 543-552.
https://doi.org/10.1080/10611860903055470
Ebbesen, M. F., Bienk, K., Deleuran, B. W. & Howard, K. (2014).
Extended circulation and accumulation of p(HPMA-co-AzMA) and accumulation in arthritic joints. Poster session presented at 41st Annual Meeting & Exposition of the Controlled Release Society, July 13-16, 2014 Chicago, USA , Chicago, United States.
Østergaard, R. H., Yan, Y., Venø, M. T.
, Dinesen, A., Kjaer-Sorensen, K., Oxvig, C., Rasmussen, J. T., Howard, K., Kjems, J., van Niel, G., Verweij, F.
& Hayashi, Y. (2022).
Extracellular Vesicle Biology Meets Zebrafish: Real-Time Visualization and RNA Profiling of Physiological Exosomes in the Bloodstream. Poster session presented at The Danish Society for Extracellular Vesicles (DSEV) 3rd Annual Symposium 2022, Roskilde, Denmark.
Hvam, M. L., Cai, Y., Dagnæs-Hansen, F., Nielsen, J., Wengel, J.
, Kjems, J. & Howard, K. (2017).
Fatty acid-modified gapmer antisense oligonucleotide and human serum albumin constructs for pharmacokinetic modulation.
Molecular Therapy,
25(7), 1710-1717.
https://doi.org/10.1016/j.ymthe.2017.05.009
Larsen, M. T., Mandrup, O. A., Schelde, K. K., Luo, Y., Sørensen, K. D., Dagnæs-Hansen, F., Cameron, J.
, Stougaard, M., Steiniche, T. & Howard, K. A. (2020).
FcRn overexpression in human cancer drives albumin recycling and cell growth: a mechanistic basis for exploitation in targeted albumin-drug designs.
Journal of Controlled Release,
322, 53-63.
https://doi.org/10.1016/j.jconrel.2020.03.004
Garcia, JP., Stein, J.
, Cai, Y., Riemers, F., Wexselblatt, E., Wengel, J., Tryfonidou, M., Yayon, A.
, Howard, K. & Creemers, L. B. (2019).
Fibrin-hyaluronic acid hydrogel-based delivery of antisense oligonucleotides for ADAMTS5 inhibition in co-delivered and resident joint cells in osteoarthritis. Journal of Controlled Release,
294, 247-258.
https://doi.org/10.1016/j.jconrel.2018.12.030
Märcher, A., Kumar, V., Andersen, V. L., El-Chami, K., Nguyen, T. J. D., Skaanning, M. K., Rudnik-Jansen, I., Nielsen, J. S., Howard, K. A., Kjems, J. & Gothelf, K. V. (2022).
Functionalized Acyclic (l)-Threoninol Nucleic Acid Four-Way Junction with High Stability In Vitro and In Vivo.
Angewandte Chemie - International Edition,
61(24), Article 202115275.
https://doi.org/10.1002/anie.202115275
Viuff, D., Antunes, F., Evans, L., Cameron, J.
, Dyrnesli, H., Ravn, B. T.
, Stougaard, M., Thiam, K., Andersen, B., Kjærulff, S.
& Howard, K. (2016).
Generation of a Double Transgenic Humanised Neonatal Fc Receptor (FcRn)/Albumin Mouse to Study the Pharmacokinetics of Albumin-linked Drugs.
Journal of Controlled Release,
223, 22-30.
https://doi.org/10.1016/j.jconrel.2015.12.019
Li, Z., Hu, Y., Chang, M.
, Howard, K., Fan, X.
, Sun, Y., Besenbacher, F. & Yu, M. (2016).
Highly porous PEGylated Bi2S3 nano-urchins as a versatile platform for in vivo triple-modal imaging, photothermal therapy and drug delivery.
Nanoscale,
8(35), 16005-16016.
https://doi.org/10.1039/C6NR03398A
Li, Z., Hu, Y., Jiang, T.
, Howard, K., Li, Y., Fan, X., Sun, Y.
, Besenbacher, F. & Yu, M. (2016).
Human serum albumin coated Prussian blue nanoparticles as pH-/thermo-triggered drug delivery vehicles for cancer thermo-chemotherapy.
Particle & Particle Systems Characterization,
33(1), 53-62.
https://doi.org/10.1002/ppsc.201500189
Hansen, I., Ebbesen, M. F., Kaspersen, L., Thomsen, T. B., Bienk, K., Cai, Y., Malle, B. M.
& Howard, K. (2017).
Hyaluronic Acid Molecular Weight-Dependent Modulation of Mucin Nanostructure for Potential Mucosal Therapeutic Applications.
Molecular Pharmaceutics,
14(7), 2359–2367.
https://doi.org/10.1021/acs.molpharmaceut.7b00236
Glud, E. N., Rasmussen, M., Zhang, Y., Mandrup, O. A., Salachan, P. V.
, Borre, M., Sørensen, K. D. & Howard, K. A. (2022).
Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design.
British Journal of Cancer,
127(12), 2186-2197.
https://doi.org/10.1038/s41416-022-01994-1
Fredsøe, J., Rasmussen, A. K. I.
, Laursen, E. B., Cai, Y., Howard, K. A., Pedersen, B. G., Borre, M., Mouritzen, P.
, Òrntoft, T. & Sørensen, K. D. (2019).
Independent Validation of a Diagnostic Noninvasive 3-MicroRNA Ratio Model (uCaP) for Prostate Cancer in Cell-Free Urine.
Clinical Chemistry,
65(4), 540-548.
https://doi.org/10.1373/clinchem.2018.296681