Aarhus University Seal / Aarhus Universitets segl

Quick Guide 3: Analyse Transitional Dynamics in Single-Molecule FRET by Hidden Markov Modelling (HMM)

Open the dynamics window in the toolbar of the main window.
In the dynamics window, run vbFRET analysis by pressing the run button in the toolbar.
A dialog will be prompted asking which molecules to run the analysis on. The analysis takes some time, so it is advised to analyse a subset of molecules at a time.
Once the analysis is finished the analysed traces will be listed in the upper listbox and all identified FRET-levels will be listed in the lower listbox.
Choose plot type of the lower axes in the popup-menu.
Export figures and data by right-clicking inside the axes.