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Gabriel Antonio Minero


Gabriel Antonio Minero

Assistant Professor Interdisciplinary Nanoscience Center

Keywords

  • Bacteriophage
  • Biofilm
  • Extracellular DNA
  • Catalytic DNAzyme
  • DNA self-replication
  • DNA self-assembly
  • DNA-binding molecules
  • Bioimaging

Member of Biofilm Group

Assistant Professor Gabriel Antonio Minero
PhD in Chemistry and Biochemistry

Shape-shifting extracellular DNA provides new functions to bacterial biofilm

Nucleic acids exist in constant equilibrium between the most thermodynamically stable B-form and various non-canonical forms. Depending on the length, sequence, nucleobase modifications and plethora of biophysical parameters, the equilibrium shifts and so does DNA shift its "shape". My research concerns investigation of the shape-shifting extracellular DNA in various biofilms - multicellular communities encapsulated into shared biopolymeric matrix. Biofilms are everywhere, and my recent studies show that biofilms can create a crowded environment, in which biomolecular processes are happening outside bacterial cells (something we would not imagine being possible). Specifically, I am looking into a novel dimension of bacteria - bacteriophage interaction and its implication in the sustainable self-replication of electrocatalytic eDNA. Extracellular G-quadruplex GQ-DNA provides a particular example of what novel functions bacteria can get thanks to eDNA. GQ-DNA binds hemin and gain peroxidase DNAzyme as well as redox activity. My studies include a lot of bioimaging using immunolabelling and fluorescent probes.

Recent publications