Nagaraj, M., Ahmed, M., Lyngsø, J., Vad, B. S.
, Bøggild, A., Fillipsen, A.
, Pedersen, J. S., Otzen, D. E. & Akbey, Ü. (2020).
Predicted Loop Regions Promote Aggregation: A Study of Amyloidogenic Domains in the Functional Amyloid FapC.
Journal of Molecular Biology,
432(7), 2232-2252.
https://doi.org/10.1016/j.jmb.2020.01.044
Pedersen, L. R. L., Nielsen, S. B., Hansted, J. G., Petersen, T. E.
, Otzen, D. E. & Sørensen, E. S. (2012).
PP3 forms stable tetrameric structures through hydrophobic interactions via the C-terminal amphipathic helix and undergoes reversible thermal dissociation and denaturation.
F E B S Journal,
279(2), 336-47.
https://doi.org/10.1111/j.1742-4658.2011.08428.x
Kurnik, M., Sahin, C., Andersen, C. B., Lorenzen, N., Giehm, L., Mohammad-Beigi, H., Jessen, C. M.
, Pedersen, J. S., Christiansen, G.
, Petersen, S. V., Staal, R., Krishnamurthy, G., Pitts, K., Reinhart, P. H.
, Mulder, F. A. A., Mente, S., Hirst, W. D.
& Otzen, D. E. (2018).
Potent α-Synuclein Aggregation Inhibitors, Identified by High-Throughput Screening, Mainly Target the Monomeric State.
Cell Chemical Biology,
25(11), 1389-1402.
https://doi.org/10.1016/j.chembiol.2018.08.005
Ghodke, S. D., Jensen, G. V., Svane, A. S. P., Weise, K.
, Søndergaard, A., Behrens, M. A.
, Pedersen, J. S., Nielsen, N. C., Pedersen, J. S., Winter, R.
& Otzen, D. (2014).
Polymorphism, metastable species and interconversion: the many states of glucagon fibrils. In V. Uversky & Y. Lyubchenko (Eds.),
Bio-nanoimaging: Protein misfolding and aggregation (pp. 373-386). Elsevier.
https://doi.org/10.1016/B978-0-12-394431-3.00034-1
Andreasen, M., Nielsen, S. B., Runager, K., Christiansen, G.
, Nielsen, N. C., Enghild, J. J. & Otzen, D. (2012).
Polymorphic Fibrillation of the Destabilized Fourth Fasciclin-1 Domain Mutant A546T of the Transforming Growth Factor-β-induced Protein (TGFBIp) Occurs through Multiple Pathways with Different Oligomeric Intermediates.
Journal of Biological Chemistry,
287(41), 34730-34742.
https://doi.org/10.1074/jbc.M112.379552
Alam, P.
, Holst, M. R., Lauritsen, L.
, Nielsen, J., Nielsen, S. S. E.
, Jensen, P. H., Brewer, J. R.
, Otzen, D. E. & Nielsen, M. S. (2022).
Polarized α-synuclein trafficking and transcytosis across brain endothelial cells via Rab7-decorated carriers.
Fluids and Barriers of the CNS,
19(1), Article 37.
https://doi.org/10.1186/s12987-022-00334-y
Najarzadeh, Z., Mohammad-Beigi, H., Pedersen, J. N., Christiansen, G., Sønderby, T. V., Shojaosadati, S. A., Morshedi, D., Strømgaard, K., Meisl, G.
, Sutherland, D., Pedersen, J. S. & Otzen, D. (2019).
Plant polyphenols inhibit functional amyloid and biofilm formation in Pseudomonas strains by directing monomers to off-pathway oligomers.
Biomolecules,
9(11), Article 659.
https://doi.org/10.3390/biom9110659
Andreasen, M., Meisl, G., Taylor, J. D., Michaels, T. C. T., Levin, A.
, Otzen, D. E., Chapman, M. R., Dobson, C. M., Matthews, S. J. & Knowles, T. P. J. (2019).
Physical Determinants of Amyloid Assembly in Biofilm Formation.
mBio,
10(1), Article e02279-18.
https://doi.org/10.1128/mBio.02279-18
Wang, H., Andersen, K. K., Sehgal, P., Hagedorn, J., Westh, P., Borch, K.
& Otzen, D. E. (2013).
pH regulation of the kinetic stability of the lipase from Thermomyces lanuginosus.
Biochemistry,
52(1), 264-276.
https://doi.org/10.1021/bi301258e
Baldry, M., Bojer, M. S., Najarzadeh, Z., Vestergaard, M.
, Meyer, R. L., Otzen, D. E. & Ingmer, H. (2020).
Phenol-Soluble Modulins Modulate Persister Cell Formation in Staphylococcus aureus.
Frontiers in Microbiology,
11, Article 573253.
https://doi.org/10.3389/fmicb.2020.573253
He, J., Becares, E. R., Thulstrup, P. W., Gamon, L. F., Pedersen, J. N.
, Otzen, D., Gourdon, P., Davies, M. J. & Hägglund, P. (2020).
Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectin.
Redox Biology,
36, Article 101631.
https://doi.org/10.1016/j.redox.2020.101631
Rusbjerg-Weberskov, C. E., Johansen, M. L.
, Nowak, J. S., Otzen, D., Pedersen, J. S., Enghild, J. J. & Nielsen, N. S. (2023).
Periostin C-Terminal Is Intrinsically Disordered and Interacts with 143 Proteins in an In Vitro Epidermal Model of Atopic Dermatitis.
Biochemistry,
62(19), 2803-2815.
https://doi.org/10.1021/acs.biochem.3c00176
Walther, R., Monge, P.
, Pedersen, A., Benderoth, A., Pedersen, J., Farzadfard, A.
, Mandrup, O., Howard, K., Otzen, D. & Zelikin, A. N. (2021).
Per-glycosylation of the Surface-Accessible Lysines: One-Pot Aqueous Route to Stabilized Proteins with Native Activity.
ChemBioChem,
22(14), 2478-2485.
https://doi.org/10.1002/cbic.202100228
Jakob, D. S., Wang, H., Zeng, G.
, Otzen, D. E., Yan, Y. & Xu, X. (2020).
Peak Force Infrared - Kelvin Probe Force Microscopy.
Angewandte Chemie International Edition,
59(37), 16083-16090.
https://doi.org/10.1002/anie.202004211
Vad, B. S., Bertelsen, K., Johansen, C. H., Pedersen, J. M.
, Skrydstrup, T., Nielsen, N. C. & Otzen, D. E. (2010).
Pardaxin permeabilizes vesicles more efficiently by pore formation than by disruption.
Biophysical Journal,
98(4), 576-85.
https://doi.org/10.1016/j.bpj.2009.08.063
Song, Y. J. C., Lundvig, D. M. S., Huang, Y., Wei, P. G., Blumbergs, P. C., Højrup, P.
, Otzen, D., Halliday, G. M.
& Jensen, P. H. (2007).
p25α relocalizes in oligodendroglia from myelin to cytoplasmic inclusions in multiple system atrophy.
American Journal of Pathology,
171(4), 1291-1303.
https://doi.org/10.2353/ajpath.2007.070201
Song, Y. J. C., Lundvig, D. M. S., Huang, Y., Gai, W. P., Blumbergs, P. C., Højrup, P.
, Otzen, D., Halliday, G. M. & Jensen, P. H. (2007).
p25a Relocalizes in Oligodendroglia from Myelin to Cytoplasmic Inclusions in Multiple System Atrophy.
Am. J. Path., (171), 1291-1303.
Singh, Y., Trautwein, C., Romani, J., Salker, M. S., Neckel, P. H., Fraccaroli, I., Abeditashi, M., Woerner, N., Admard, J., Dhariwal, A., Dueholm, M. K. D., Schäfer, K. H., Lang, F.
, Otzen, D. E., Lashuel, H. A., Riess, O. & Casadei, N. (2023).
Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease.
Molecular Neurodegeneration,
18(1), Article 44.
https://doi.org/10.1186/s13024-023-00628-1
Mohammad-Beigi, H., Aliakbari, F., Sahin, C., Lomax, C., Tawfike, A., P. Schafer, N., Amiri-Nowdijeh, A.
, Eskandari, H., Møller, I. M., Hosseini-Mazinani, M., Christiansen, G., Ward, J. L., Morshedi, D.
& Otzen, D. (2019).
Oleuropein derivatives from olive fruit extracts reduce α-synuclein fibrillation and oligomer toxicity.
Journal of Biological Chemistry,
294(11), 4215-4232.
https://doi.org/10.1074/jbc.RA118.005723
Deva, T., Lorenzen, N., Vad, B. S.
, Petersen, S. V., Thørgersen, I.
, Enghild, J. J., Kristensen, T.
& Otzen, D. (2013).
Off-pathway aggregation can inhibit fibrillation at high protein concentrations.
BBA General Subjects,
1834(3), 677-687.
https://doi.org/10.1016/j.bbapap.2012.12.020
Triano, I., Barrera, F. N., Renart, M. L., Molina, M. L., Fernández-Ballester, G., Poveda, J. A., Fernández, A. M., Encinar, J. A., Ferrer-Montiel, A. V.
, Otzen, D. & González-Ros, J. M. (2010).
Occupancy of nonannular lipid binding sites on KcsA greatly increases the stability of the tetrameric protein.
Biochemistry,
49(25), 5397-5404.
https://doi.org/10.1021/bi1003712
Alijanvand, S. H.
, Christensen, M. H., Christiansen, G., Harikandei, K. B., Salehi, P.
, Schiøtt, B., Moosavi-Movahedi, A. A.
& Otzen, D. E. (2020).
Novel noscapine derivatives stabilize the native state of insulin against fibrillation.
International Journal of Biological Macromolecules,
147, 98-108.
https://doi.org/10.1016/j.ijbiomac.2020.01.061
Knudsen, L. J., Nielsen, S. D.-H., Rauh, V.
, Otzen, D. & Larsen, L. B. (2019).
New Lactase Enzymes. Abstract from Sandbjerg Seminar 2019, Sønderborg, Denmark.
Kulminskaya, N. V., Yoshimura, Y., Runager, K., Sørensen, C. S., Bjerring, M.
, Andreasen, M., Otzen, D. E., Enghild, J. J., Nielsen, N. C. & Mulder, F. A. A. (2016).
Near-complete 1H, 13C, 15N resonance assignments of dimethylsulfoxide-denatured TGFBIp FAS1-4 A546T.
Biomolecular N M R Assignments,
10(1), 25-29.
https://doi.org/10.1007/s12104-015-9630-2
Runager, K., Underhaug, J.
, Basaiawmoit, R. V., Valnickova, Z., Kristensen, T.
, Otzen, D., Klintworth, G. K.
, Nielsen, N. C. & Enghild, J. J. (2009).
Naturally Occurring Mutations Alter the Structure and Stability of the Fourth Fasciclin Domain of the Transforming Growth Factor Beta Induced Protein (TGFBIp).
Investigative Ophthalmology & Visual Science, (E-Abstract 1016).
Huma, Z.-E., Javed, I., Zhang, Z., Bilal, H., Sun, Y., Hussain, S. Z., Davis, T. P.
, Otzen, D. E., Landersdorfer, C. B., Ding, F., Hussain, I. & Ke, P. C. (2020).
Nanosilver Mitigates Biofilm Formation via FapC Amyloidosis Inhibition.
Small (Weinheim an der Bergstrasse, Germany),
16(21), Article 1906674.
https://doi.org/10.1002/smll.201906674
Mortensen, H. G., Madsen, J. K., Andersen, K. K.
, Vosegaard, T., Deen, G. R.
, Otzen, D. E. & Pedersen, J. S. (2017).
Myoglobin and α-Lactalbumin Form Smaller Complexes with the Biosurfactant Rhamnolipid Than with SDS.
Biophysical Journal,
113(12), 2621-2633.
https://doi.org/10.1016/j.bpj.2017.10.024
Underhaug, J., Koldsø, H., Runager, K., Nielsen, J. T., Sørensen, C. S., Kristensen, T.
, Otzen, D., Karring, H., Malmendal, A.
, Schiøtt, B., Enghild, J. J. & Nielsen, N. C. (2013).
Mutation in transforming growth factor beta induced protein associated with granular corneal dystrophy type 1 reduces the proteolytic susceptibility through local structural stabilization.
BBA General Subjects,
1834(12), 2812–2822.
https://doi.org/10.1016/j.bbapap.2013.10.008
Ferreira, N.
, Gram, H., Sorrentino, Z. A.
, Gregersen, E., Schmidt, S. I.
, Reimer, L., Betzer, C., Perez-Gozalbo, C., Beltoja, M., Nagaraj, M., Wang, J.
, Nowak, J. S., Dong, M., Willén, K., Cholak, E., Bjerregaard-Andersen, K., Mendez, N., Rabadia, P., Shahnawaz, M.
... Jensen, P. H. (2021).
Multiple system atrophy-associated oligodendroglial protein p25α stimulates formation of novel α-synuclein strain with enhanced neurodegenerative potential.
Acta Neuropathologica,
142, 87-115.
https://doi.org/10.1007/s00401-021-02316-0
Nielsen, S. B., Yde, P., Giehm, L., Sundbye, S., Christiansen, G., Matthiesen, J., Jensen, M. H.
, Jensen, P. H. & Otzen, D. E. (2012).
Multiple Roles of Heparin in the Aggregation of p25α.
Journal of Molecular Biology.
https://doi.org/10.1016/j.jmb.2012.01.050
Aliakbari, F., Mohammad-Beigi, H., Abbasi, S., Rezaei-Ghaleh, N., Lermyte, F., Parsafar, S., Becker, S., Tafreshi, A. P., O'Connor, P. B., Collingwood, J. F., Christiansen, G.
, Sutherland, D. S., Jensen, P. H., Morshedi, D.
& Otzen, D. E. (2021).
Multiple Protective Roles of Nanoliposome-Incorporated Baicalein against Alpha-Synuclein Aggregates.
Advanced Functional Materials,
31(7), Article 2007765.
https://doi.org/10.1002/adfm.202007765
Matouschek, A.
, Otzen, D. E., Itzhaki, L. S., Jackson, S. E. & Fersht, A. R. (1995).
Movement of the Position of the Transition State in Protein Folding.
Biochemistry,
34(41), 13656-13662.
https://doi.org/10.1021/bi00041a047
Sønderby, T. V., Zou, Y., Wang, P., Wang, C.
& Otzen, D. E. (2022).
Molecular-level insights into the surface-induced assembly of Functional Bacterial Amyloid.
Biophysical Journal,
121(18), 3422-3434.
https://doi.org/10.1016/j.bpj.2022.08.013
Poghosyan, A. H., Schafer, N., Lyngsø, J., Shahinyan, A. A.
, Pedersen, J. S. & Otzen, D. (2019).
Molecular dynamics study of ACBP denaturation in alkyl sulfates demonstrates possible pathways of unfolding through fused surfactant clusters.
Protein Engineering, Design and Selection,
32(4), 175-190. Article gzz037.
https://doi.org/10.1093/protein/gzz037
Larsen, K., Bæk, R., Sahin, C., Kjær, L., Christiansen, G.
, Nielsen, J., Farajzadeh, L.
& Otzen, D. E. (2021).
Molecular characteristics of porcine alpha-synuclein splicing variants.
Biochimie,
180, 121-133.
https://doi.org/10.1016/j.biochi.2020.10.019
Andreasen, M., Nielsen, S. B., Mittag, T., Bjerring, M., Nielsen, J. T., Zhang, S., Nielsen, E. H., Jeppesen, M., Christiansen, G.
, Besenbacher, F., Dong, M., Nielsen, N. C., Skrydstrup, T. & Otzen, D. E. (2012).
Modulation of fibrillation of hIAPP core fragments by chemical modification of the peptide backbone.
B B A - Proteins and Proteomics,
1824(2), 274-85.
https://doi.org/10.1016/j.bbapap.2011.10.014
Sehgal, P., Bang Nielsen, S., Pedersen, S., Wimmer, R.
& Otzen, D. (2007).
Modulation of cutinase structure and stability by phospholipid detergents.
Biochim. Biophys. Acta., (1774), 1544-1554.
Umakoshi, H., Persson, J., Kroon, M., Johansson, H. O.
, Otzen, D. E., Kuboi, R. & Tjerneld, F. (2000).
Model process for separation based on unfolding and refolding of chymotrypsin inhibitor 2 in thermoseparating polymer two-phase systems.
Journal of Chromatography B: Biomedical Sciences and Applications,
743(1-2), 13-19.
https://doi.org/10.1016/S0378-4347(00)00190-0
Martins, P. M., Navarro, S., Silva, A., Pinto, M. F., Sárkány, Z., Figueiredo, F., Pereira, P. J. B., Pinheiro, F., Bednarikova, Z., Burdukiewicz, M., Galzitskaya, O. V., Gazova, Z., Gomes, C. M., Pastore, A., Serpell, L. C., Skrabana, R., Smirnovas, V., Ziaunys, M.
, Otzen, D. E. ... Macedo-Ribeiro, S. (2020).
MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments.
Frontiers in Molecular Neuroscience,
13, Article 582488.
https://doi.org/10.3389/fnmol.2020.582488